Patterson M, Healy G R, Shabot J M
Gastroenterology. 1980 Jan;78(1):136-41.
The diagnosis of amoebiasis presents problems, particularly if one relies on finding the organism. Thus, serologic tests are expedient. A gel diffusion precipitin test (GDP), commercially available, simple to perform, and inexpensive, was compared with the indirect hemagglutination test (IHA). 257 Patients' sera were tested; 14 had amoebic colitis, 21 had amoebic liver abscess, 63 had suspected amoebic liver abscess, and 46 had inflammatory bowel disease. GDP tests were positive in 85% of amoebic colitis and 95% of amoebic liver abscess patients; IHA was positive in 91% of amoebic colitis and 94% of abscess patients. Within 6 mo, GDP tests became negative in 66% of patients. IHA tests were observed positive up to 20 yr. The performance characteristics of diagnostic methods in amoebiasis, fecal examination, IHA and GDP, show serologic tests have superior sensitivity and predictive value in recognizing invasive disease.
阿米巴病的诊断存在问题，尤其是在依赖发现病原体的情况下。因此，血清学检测较为便利。将一种凝胶扩散沉淀试验（GDP）与间接血凝试验（IHA）进行了比较，GDP试验市售可得、操作简单且成本低廉。对257例患者的血清进行了检测；其中14例患有阿米巴结肠炎，21例患有阿米巴肝脓肿，63例疑似阿米巴肝脓肿，46例患有炎症性肠病。GDP试验在85%的阿米巴结肠炎患者和95%的阿米巴肝脓肿患者中呈阳性；IHA在91%的阿米巴结肠炎患者和94%的脓肿患者中呈阳性。在6个月内，66%的患者GDP试验转为阴性。IHA试验在长达20年的时间里都呈阳性。在阿米巴病中，粪便检查、IHA和GDP等诊断方法的性能特征表明，血清学检测在识别侵袭性疾病方面具有更高的敏感性和预测价值。
