Vinayak V K, Talwar G P, Tandon B N, Mohapatra L N
J Trop Med Hyg. 1975 Mar;78(3):63-6.
The double diffusion precipitinin test for amoebiasis, has been evaluated in invasive amoebiasis. It gave positive percentages of 91-3 and 90-0 in cases of amoebic liver abscess and amoebic dysentery. False positive rates of 8-0 per cent and 0-0 per cent were obtained in miscellaneous non-amoebic disorders and in apparently healthy subjects, respectively. Its comparison with the indirect haemagglutination test revealed it to be slightly less sensitive but more specific. The agar gel precipitinin test became positive whenever the indirect haemagglutination titres were 1:256 or more. The adsorption studies revealed that both tests detected different types of antibodies. The use of the gel diffusion test is recommended as a routine method for amoebiasis as it is simple to perform in areas where sophisticated laboratory methods are not available.
用于阿米巴病的双向扩散沉淀素试验已在侵袭性阿米巴病中进行了评估。在阿米巴肝脓肿和阿米巴痢疾病例中，其阳性率分别为91.3%和90.0%。在各种非阿米巴病疾病和明显健康的受试者中，假阳性率分别为8.0%和0.0%。与间接血凝试验相比，发现它的敏感性略低，但特异性更高。每当间接血凝滴度为1:256或更高时，琼脂凝胶沉淀素试验就呈阳性。吸附研究表明，两种试验检测的是不同类型的抗体。由于在没有精密实验室方法的地区该试验操作简单，因此建议将凝胶扩散试验作为阿米巴病的常规方法使用。
