Bunin K V, Firsanov V I, Parfenova N N, Krartsov E G, Efimor D D
Zh Mikrobiol Epidemiol Immunobiol. 1978 Jun(6):89-92.
The authors studied the comparative antigenic activity in the indirect hemagglutination test (IHAT) of erythrocytic diagnostic agents sensitized with various doses of cholera O-antigen. A definite dependence of the IHAT results on the avidity degree of the antibodies determined in the serum was demonstrated. To ascertain the maximal levels of highly avid antibodies (in the hyperimmune cholera sera) it is possible to use antigen doses exceeding the optimal sensitizing ones. In examination of less avid antibodies (for example in the blood sera of cholera patients) the diagnostic agent in such doses displayed decreased IHAT titres. To increase the reliability of the IHAT results it is recommended to choose the sensitizing antigen doses for the preparation of the diagnostic agent to be used in the IHAT with consideration to the avidity of antibodies examined in the blood sera.
作者研究了用不同剂量霍乱O抗原致敏的红细胞诊断剂在间接血凝试验（IHAT）中的比较抗原活性。结果表明，IHAT结果与血清中抗体的亲和力程度存在明确的相关性。为了确定高亲和力抗体的最大水平（在超免疫霍乱血清中），可以使用超过最佳致敏剂量的抗原剂量。在检测亲和力较低的抗体时（例如霍乱患者的血清），这种剂量的诊断剂在IHAT中的滴度会降低。为了提高IHAT结果的可靠性，建议在制备用于IHAT的诊断剂时，根据血清中检测到的抗体亲和力来选择致敏抗原剂量。
