Sharief Y, Reich C F, Bonar R A
Invest Urol. 1980 Mar;17(5):390-4.
Swine bladder epithelial cells in culture were treated with 4-nitroquinoline-1-oxide (4NQO) for 4 hr with three different concentrations to examine dose-dependent effects. To study time course effects, they were treated with one concentration for 4 hr and then incubated in 4NQO-free medium for three different periods before harvesting. Three different effects were recorded: (i) a suppression of mitotic activity that was dose-dependent and which continued for more than 30 hr posttreatment; (ii) no marked changes in the proportions of different ploidy classes; and (iii) chromatid gaps, breaks, and exchanges that were dose-dependent. This clastogenic effect decreased with increasing time after treatment.
培养的猪膀胱上皮细胞用三种不同浓度的4-硝基喹啉-1-氧化物（4NQO）处理4小时，以检查剂量依赖性效应。为了研究时间进程效应，将它们用一种浓度处理4小时，然后在收获前在无4NQO的培养基中孵育三个不同的时间段。记录了三种不同的效应：（i）有丝分裂活性的抑制，其具有剂量依赖性，并且在处理后持续超过30小时；（ii）不同倍体类别的比例没有明显变化；（iii）染色单体间隙、断裂和交换，其具有剂量依赖性。这种致断裂效应随着处理后时间的增加而降低。
