人工晶状体消毒研究：氢氧化钠对枯草芽孢杆菌孢子的影响

Studies of intraocular lens sterilization: the effect of NaOH on b. subtilis spores.

作者信息

Galin M A, Turkish L

出版信息

J Am Intraocul Implant Soc. 1980 Jan;6(1):18-20. doi: 10.1016/s0146-2776(80)80100-5.

Abstract

The Ridley method for intraocular lens sterilization employs 10% NaOH for one hour followed by long-term storage in 0.1% NaOH. Ten percent NaOH for one hour was found to be ineffective when 106 B. subtilis spores were used for study in the presence of a looped lens system. A three-hour period was found to be effective. Similar concentrations of organisms were not killed by 0.1% NaOH even after 30 days. Consequently, the standard Ridley method would be inadequate for looped lens systems and would not fulfill the requirements for terminal sterilization. Teh percent NaOH for three hours would appear to be an acceptable terminal sterilization method.

摘要

用于人工晶状体灭菌的里德利方法是先用10%的氢氧化钠处理一小时，然后长期保存在0.1%的氢氧化钠中。当使用106个枯草芽孢杆菌孢子在有环状晶状体系统的情况下进行研究时，发现10%的氢氧化钠处理一小时无效。发现三小时的处理时间是有效的。即使在30天后，0.1%的氢氧化钠也不能杀死类似浓度的微生物。因此，标准的里德利方法对于环状晶状体系统是不够的，不能满足最终灭菌的要求。用氢氧化钠处理三小时似乎是一种可接受的最终灭菌方法。