Jakimiak B, Bielicka A
Zakład Zwalczania Skazeń Biologicznych Państwowego Zakładu Higieny w Warszawie.
Rocz Panstw Zakl Hig. 1994;45(3):241-8.
It was found the correlation between the storage time of biological indicators and their usefulness for monitoring of gas sterilization with ethylene oxide (EO). Bacillus subtilis var niger ATTC 9372 was used as a test organism. Two kinds of investigations were done: observation of bacterial growing or lack of growing after 1 hour exposition of storage tests in EO (EO concentration- 750 mg/l, temperature +/- 50 degrees C, (humidity-40%) and examination of drop in number of spores on strip tests after storage time. The results show that sterilization efficacy may be monitored using such spore strip tests up to six months of storage.
研究发现了生物指示剂的储存时间与其对环氧乙烷（EO）气体灭菌监测的有效性之间的相关性。使用枯草芽孢杆菌黑色变种ATCC 9372作为试验菌株。进行了两种调查：在EO中进行储存试验1小时暴露后观察细菌生长或不生长情况（EO浓度-750毫克/升，温度±50摄氏度，湿度-40%），以及储存时间后条带试验中孢子数量的下降检查。结果表明，使用此类孢子条带试验监测灭菌效果，储存时间可达六个月。
