Woodard L F, Toone N
Am J Vet Res. 1980 Jan;41(1):114-6.
Three Brucella abortus strain 45/20 antigen preparations were compared with regard to their chemical composition and allergenic potential. A popular method of extracting soluble antigens from whole cells with hypertonic saline solution and ethanol precipitation was compared with the method of trichloroacetic acid (TCA) or ammonium sulfate (AS) precipitation of antigens released into solution by ultrasonic cell disruption. Biochemical analysis of the preparations revealed that TCA and AS precipitates had considerably more protein and DNA and less carbohydrates and RNA than did the saline-ethanol extracts. Strain 19-vaccinated cattle were simultaneously skin tested with the three antigen preparations which had been standardized to an equal protein content. Significant (P less than 0.01) delayed hypersensitivity reactions were observed in response to all three antigen preparations. However, TCA-prepared allergens were more active in eliciting delayed hypersensitivity (P less tha 0.05) than were the two other allergens. Increased agglutinating antibodies were not observed in response to the skin-test reagents.
对三种流产布鲁氏菌45/20菌株抗原制剂的化学成分和致敏潜力进行了比较。将一种用高渗盐溶液从全细胞中提取可溶性抗原并进行乙醇沉淀的常用方法，与通过超声细胞破碎将抗原释放到溶液中后用三氯乙酸（TCA）或硫酸铵（AS）沉淀的方法进行了比较。制剂的生化分析表明，与盐-乙醇提取物相比，TCA和AS沉淀物中的蛋白质和DNA含量要多得多，而碳水化合物和RNA含量则少得多。用三种已标准化至相同蛋白质含量的抗原制剂对19号菌株疫苗接种的牛同时进行皮肤试验。对所有三种抗原制剂均观察到显著（P<0.01）的迟发型超敏反应。然而，TCA制备的变应原在引发迟发型超敏反应方面比其他两种变应原更具活性（P<0.05）。对皮肤试验试剂未观察到凝集抗体增加。
