Woodard L F, Toone N M, McLaughlin C A
Can J Comp Med. 1980 Oct;44(4):453-5.
A purified protein derivative-live preparation of Brucella abortus strain 45/20 was tested for immunogenic properties either alone, after lipid conjugation, or in association with defined adjuvants. The adjuvants were trehalose dimycolate (cord factor), isolated from wax D of mycobacteria and murmyl dipeptide (N-acetyl-muramyl-L-alamyl-D-isoglutamine), a synthetic glycopeptide analog of peptidoglycan subunits found in many bacterial cell walls and wax D of mycobacteria. Guinea pigs were intradermally inoculated with a single injection of the vaccine preparations eight weeks before intramuscular challenge with B. abortus, strain 2308. None of the purified protein derivative-like preparations were as effective in the prevention of splenic infections with B. abortus as were killed whole cells of strain 45/20 in Freund's complete adjuvant. Whole cells in Freund's complete adjuvant were able to reduce mean colony counts by 97% (P = 0.02), while purified protein derivative-like vaccines were able to reduce mean colony counts by only 32 to 61% as compared to control animals. Results suggest that purified protein derivative-like preparations have limited immunogenic properties under present test conditions.
对流产布鲁氏菌45/20株的纯化蛋白衍生物-活菌制剂单独、脂质缀合后或与特定佐剂联合使用时的免疫原性进行了测试。佐剂为海藻糖二霉菌酸酯（索状因子），从分枝杆菌的蜡质D中分离得到，以及胞壁酰二肽（N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺），一种在许多细菌细胞壁和分枝杆菌蜡质D中发现的肽聚糖亚基的合成糖肽类似物。在豚鼠经肌肉注射流产布鲁氏菌2308株进行攻击前八周，对其进行皮内单次注射疫苗制剂。没有一种纯化蛋白衍生物样制剂在预防流产布鲁氏菌脾脏感染方面像弗氏完全佐剂中的45/20株死全菌那样有效。弗氏完全佐剂中的全菌能够使平均菌落数减少97%（P = 0.02），而与对照动物相比，纯化蛋白衍生物样疫苗只能使平均菌落数减少32%至61%。结果表明，在目前的测试条件下，纯化蛋白衍生物样制剂的免疫原性有限。
