Ieiri T, Chen H T, Campbell G A, Meites J
Endocrinology. 1980 May;106(5):1568-70. doi: 10.1210/endo-106-5-1568.
The effects of naloxone hydrochloride and morphine sulfate on the proestrous surge of PRL and gonadotropins (LH and FSH) were investigated in normal cycling Sprague-Dawley rats. Blood samples (0.45-0.50 ml) were withdrawn without anesthesia every 20 min from 1400-2000 h through an atrial cannula implanted the same morning. RIA revealed that a single iv injection of naloxone (0.2 mg/kg) at 1400 h completely suppressed the surge of PRL, and this was reversed by a concomitant injection of morphine (10 mg/kg). Morphine itself did not alter the peak of the PRL surge. Morphine suppressed only the early phase of the LH surge, and this was reversed by naloxone. Naloxone alone did not change the peak of the LH surge but maintained higher levels than controls during the declining phase. The FSH surge was not altered by either morphine or naloxone. These results suggest that endogenous opioid peptides may have a role in regulating the PRL and LH surges during proestrus in the rat.
在正常发情周期的斯普拉格-道利大鼠中，研究了盐酸纳洛酮和硫酸吗啡对促乳素（PRL）以及促性腺激素（促黄体生成素LH和促卵泡生成素FSH）发情前期激增的影响。通过当天上午植入的心房插管，于14:00至20:00期间每隔20分钟在无麻醉状态下采集血样（0.45 - 0.50毫升）。放射免疫分析显示，14:00单次静脉注射纳洛酮（0.2毫克/千克）可完全抑制PRL的激增，同时注射吗啡（10毫克/千克）可使其恢复。吗啡本身并未改变PRL激增的峰值。吗啡仅抑制LH激增的早期阶段，而纳洛酮可使其恢复。单独使用纳洛酮不会改变LH激增的峰值，但在下降阶段维持的水平高于对照组。吗啡和纳洛酮均未改变FSH的激增。这些结果表明，内源性阿片肽可能在调节大鼠发情前期PRL和LH的激增中发挥作用。
