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蓝细菌水华束丝藻6308中藻青素颗粒的结构与组成

Structure and composition of cyanophycin granules in the cyanobacterium Aphanocapsa 6308.

作者信息

Allen M M, Weathers P J

出版信息

J Bacteriol. 1980 Feb;141(2):959-62. doi: 10.1128/jb.141.2.959-962.1980.

DOI:10.1128/jb.141.2.959-962.1980
PMID:6767696
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC293718/
Abstract

Cyanophycin granules in the unicellular cyanobacterium Aphanocapsa 6308 were examined with the electron microscope in both thin section and by freeze-fracture techniques. Purified granules were examined with the electron microscope, by arginine determinations, by chromatography, and by elemental analysis. They are similar in ultrastructure and composition to those isolated from the nitrogen-fixing cyanobacterium Anabaena cylindrica, consisting of equal molar quantities of L-arginine and L-aspartic acid.

摘要

运用超薄切片和冷冻蚀刻技术，通过电子显微镜对单细胞蓝藻隐球藻6308中的藻青素颗粒进行了检测。运用电子显微镜、精氨酸测定、色谱分析和元素分析等方法对纯化后的颗粒进行了检测。这些颗粒在超微结构和组成上与从固氮蓝藻柱孢鱼腥藻中分离出的颗粒相似，由等摩尔量的L-精氨酸和L-天冬氨酸组成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ede/293718/0de47ad2f31d/jbacter00563-0531-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ede/293718/f459f1a63853/jbacter00563-0530-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ede/293718/0de47ad2f31d/jbacter00563-0531-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ede/293718/f459f1a63853/jbacter00563-0530-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ede/293718/0de47ad2f31d/jbacter00563-0531-a.jpg

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本文引用的文献

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Proc Natl Acad Sci U S A. 1971 Feb;68(2):265-7. doi: 10.1073/pnas.68.2.265.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Ultrastructure of the cell wall and cell division of unicellular blue-green algae.单细胞蓝藻细胞壁的超微结构及细胞分裂
藻青素代谢基因的生物信息学和催化藻青素降解最后一步的多功能异天冬酰二肽酶的特性。
Sci Rep. 2023 May 23;13(1):8314. doi: 10.1038/s41598-023-34587-w.
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Discovery of cyanophycin dipeptide hydrolase enzymes suggests widespread utility of the natural biopolymer cyanophycin.发现藻青素二肽水解酶表明天然生物聚合物藻青素具有广泛的用途。
Proc Natl Acad Sci U S A. 2023 Feb 21;120(8):e2216547120. doi: 10.1073/pnas.2216547120. Epub 2023 Feb 17.
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Appl Environ Microbiol. 2017 Sep 15;83(19). doi: 10.1128/AEM.01068-17. Print 2017 Oct 1.
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