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用于可食用蓝细菌的模拟微重力装置的开发与实施。

Development and implementation of a simulated microgravity setup for edible cyanobacteria.

作者信息

Ellena Gabriele, Fahrion Jana, Gupta Surya, Dussap Claude-Gilles, Mazzoli Arianna, Leys Natalie, Mastroleo Felice

机构信息

Microbiology Unit, Nuclear Medical Applications, Belgian Nuclear Research Center SCK CEN, Mol, Belgium.

Università degli Studi di Napoli Federico II, Department of Biology, Complesso Universitario di Monte Sant'Angelo, Edificio 7, Via Cinthia, I-80126, Napoli, Italy.

出版信息

NPJ Microgravity. 2024 Oct 25;10(1):99. doi: 10.1038/s41526-024-00436-x.

DOI:10.1038/s41526-024-00436-x
PMID:39455588
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11511917/
Abstract

Regenerative life support systems for space crews recycle waste into water, food, and oxygen using different organisms. The European Space Agency's MELiSSA program uses the cyanobacterium Limnospira indica PCC8005 for air revitalization and food production. Before space use, components' compatibility with reduced gravity was tested. This study introduced a ground analog for microgravity experiments with oxygenic cyanobacteria under continuous illumination, using a random positioning machine (RPM) setup. L. indica PCC8005 grew slower under low-shear simulated microgravity, with proteome analysis revealing downregulation of ribosomal proteins, glutamine synthase, and nitrate uptake transporters, and upregulation of gas vesicle, photosystem I and II, and carboxysome proteins. Results suggested inhibition due to high oxygen partial pressure, causing carbon limitation when cultivated in low-shear simulated microgravity. A thicker stagnant fluid boundary layer reducing oxygen release in simulated microgravity was observed. These findings validate this RPM setup for testing the effects of non-terrestrial gravity on photosynthetic microorganisms.

摘要

用于太空船员的再生生命支持系统利用不同生物体将废物再循环为水、食物和氧气。欧洲航天局的MELiSSA计划使用蓝细菌印度螺旋藻PCC8005进行空气再生和食物生产。在太空使用之前，测试了组件与微重力的兼容性。本研究引入了一种地面模拟装置，用于在连续光照下对产氧蓝细菌进行微重力实验，采用随机定位机（RPM）设置。印度螺旋藻PCC8005在低剪切模拟微重力下生长较慢，蛋白质组分析显示核糖体蛋白、谷氨酰胺合成酶和硝酸盐摄取转运蛋白下调，而气泡、光系统I和II以及羧酶体蛋白上调。结果表明，高氧分压导致抑制作用，在低剪切模拟微重力下培养时导致碳限制。观察到在模拟微重力下较厚的停滞流体边界层减少了氧气释放。这些发现验证了这种RPM设置用于测试非地球重力对光合微生物的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/bbe5ee73a108/41526_2024_436_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/97b08880ac27/41526_2024_436_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/9f639b0e7cec/41526_2024_436_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/f11ebe33c184/41526_2024_436_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/d6a28e7c5aa4/41526_2024_436_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/bdcb61129f4e/41526_2024_436_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/b1ad7d6d5d80/41526_2024_436_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/bbe5ee73a108/41526_2024_436_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/97b08880ac27/41526_2024_436_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/19cb3c51a703/41526_2024_436_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/66cc0425f98b/41526_2024_436_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/9f639b0e7cec/41526_2024_436_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/f11ebe33c184/41526_2024_436_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/d6a28e7c5aa4/41526_2024_436_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/bdcb61129f4e/41526_2024_436_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/b1ad7d6d5d80/41526_2024_436_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e4b/11511917/bbe5ee73a108/41526_2024_436_Fig9_HTML.jpg

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