短小芽孢杆菌的trpE基因克隆对枯草芽孢杆菌谷氨酰胺酰胺转移酶亚基的调控
Regulation of a glutamine amidotransferase subunit from Bacillus subtilis by a cloned trpE gene from Bacillus pumilus.
作者信息
Kane J F, Hill R J
出版信息
J Bacteriol. 1980 Feb;141(2):993-5. doi: 10.1128/jb.141.2.993-995.1980.
Abstract
The influence of a cloned trpE (trpE+p) gene from Bacillus pumilus on the expression of the gat locus in Bacillus subtilis was examined. The trpE gene was regulated by tryptophan and the mtr locus, which specifies the presumed aporepressor. The specific activity of subunit G varied directly with the level of subunit Ep, and the heterologous EpG complex that was formed was stable to gel filtration.
摘要
研究了短小芽孢杆菌的克隆色氨酸操纵子E基因（trpE+p）对枯草芽孢杆菌gat基因座表达的影响。trpE基因受色氨酸和mtr基因座调控，mtr基因座编码假定的无辅基阻遏物。亚基G的比活性与亚基Ep的水平直接相关，形成的异源EpG复合物在凝胶过滤中稳定。
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