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短小芽孢杆菌的trpE基因克隆对枯草芽孢杆菌谷氨酰胺酰胺转移酶亚基的调控

Regulation of a glutamine amidotransferase subunit from Bacillus subtilis by a cloned trpE gene from Bacillus pumilus.

作者信息

Kane J F, Hill R J

出版信息

J Bacteriol. 1980 Feb;141(2):993-5. doi: 10.1128/jb.141.2.993-995.1980.

DOI:10.1128/jb.141.2.993-995.1980
PMID:6767699
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC293734/
Abstract

The influence of a cloned trpE (trpE+p) gene from Bacillus pumilus on the expression of the gat locus in Bacillus subtilis was examined. The trpE gene was regulated by tryptophan and the mtr locus, which specifies the presumed aporepressor. The specific activity of subunit G varied directly with the level of subunit Ep, and the heterologous EpG complex that was formed was stable to gel filtration.

摘要

研究了短小芽孢杆菌的克隆色氨酸操纵子E基因（trpE+p）对枯草芽孢杆菌gat基因座表达的影响。trpE基因受色氨酸和mtr基因座调控，mtr基因座编码假定的无辅基阻遏物。亚基G的比活性与亚基Ep的水平直接相关，形成的异源EpG复合物在凝胶过滤中稳定。

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引用本文的文献

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Effect of glutamine on enzymes of nitrogen metabolism in Bacillus subtilis.谷氨酰胺对枯草芽孢杆菌氮代谢酶的影响。
J Bacteriol. 1981 Feb;145(2):768-74. doi: 10.1128/jb.145.2.768-774.1981.

本文引用的文献

1
Control of tryptophan biosynthesis by the methyltryptophan resistance gene in Bacillus subtilis.枯草芽孢杆菌中甲基色氨酸抗性基因对色氨酸生物合成的调控
J Bacteriol. 1971 Jan;105(1):38-45. doi: 10.1128/jb.105.1.38-45.1971.
2
A biochemical basis for apparent abortive transformation in Bacillus subtilis.枯草芽孢杆菌中明显的流产转化的生化基础。
Genetics. 1968 Dec;60(4):707-17. doi: 10.1093/genetics/60.4.707.
3
Metabolic interlock. The dual function of a folate pathway gene as an extra-operonic gene of tryptophan biosynthesis.代谢连锁。叶酸途径基因作为色氨酸生物合成的额外操纵子基因的双重功能。
J Biol Chem. 1972 Mar 10;247(5):1587-96.
4
Homologous and hybrid complexes of anthranilate synthase from Bacillus species.芽孢杆菌属邻氨基苯甲酸合酶的同源及杂合复合物
J Bacteriol. 1974 Jul;119(1):220-7. doi: 10.1128/jb.119.1.220-227.1974.
5
Mapping of the 5-methyltryptophan resistance locus in Bacillus subtilis.枯草芽孢杆菌中5-甲基色氨酸抗性位点的定位
J Bacteriol. 1974 Jan;117(1):315-7. doi: 10.1128/jb.117.1.315-317.1974.
6
Enzymes of the tryptophan pathway in three Bacillus species.三种芽孢杆菌属细菌中色氨酸途径的酶
J Bacteriol. 1973 Nov;116(2):685-93. doi: 10.1128/jb.116.2.685-693.1973.
7
Anthranilate synthase from Bacillus subtilis. The role of a reduced subunit X in aggregate formation and amidotransferase activity.来自枯草芽孢杆菌的邻氨基苯甲酸合酶。还原型亚基X在聚集体形成和氨基转移酶活性中的作用。
J Biol Chem. 1975 Jun 25;250(12):4462-9.
8
Molecular cloning of genetically active fragments of Bacillus DNA in Bacillus subtilis and properties of the vector plasmid pUB110.枯草芽孢杆菌中芽孢杆菌属DNA基因活性片段的分子克隆及载体质粒pUB110的特性
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1423-7. doi: 10.1073/pnas.75.3.1423.
9
Regulation of a common amidotransferase subunit.一种常见酰胺转移酶亚基的调控
J Bacteriol. 1977 Nov;132(2):419-25. doi: 10.1128/jb.132.2.419-425.1977.
10
Isolation and characterization of four plasmids from Bacillus subtilis.枯草芽孢杆菌中四种质粒的分离与鉴定
J Bacteriol. 1977 Mar;129(3):1487-94. doi: 10.1128/jb.129.3.1487-1494.1977.