McMahon M E, Katze J R, Jensen T
J Bacteriol. 1980 Mar;141(3):1239-45. doi: 10.1128/jb.141.3.1239-1245.1980.
Fifteen aminoacyl-transfer ribonucleic acids (tRNA's) from vegetative cells (trophozoites) and mature cysts of Acanthamoeba castellanii were compared by reversed-phase 5 chromatography. Little or no differences were detected in reversed-phase 5 chromatography elution profiles of alanyl-, arginyl-, isoleucyl-, phenylalanyl-, prolyl-, seryl-, threonyl-, tryptophanyl- and valyl-tRNA's. Significant differences in the relative proportions of isoaccepting species of leucyl-, lysyl-, methionyl-, aspartyl-, histidyl-, and tyrosyl-tRNA's were observed. Based upon the criterion of cyanogen bromide reactivity with the modified nucleoside queuosine, the content of queuosine in aspartyl-tRNA of A, castellanii is significantly greater in mature cysts than in trophozoites. The similarity of change in reversed-phase 5 chromatography elution profiles of aspartyl-, histidyl-, and tyrosyl-tRNA suggests that a common mechanism is responsible for alterations in the chromatographic patterns.
采用反相5色谱法对卡氏棘阿米巴滋养体和成熟包囊的15种氨酰基转移核糖核酸（tRNA）进行了比较。丙氨酰 - 、精氨酰 - 、异亮氨酰 - 、苯丙氨酰 - 、脯氨酰 - 、丝氨酰 - 、苏氨酰 - 、色氨酰 - 和缬氨酰 - tRNA的反相5色谱洗脱图谱中未检测到明显差异或根本没有差异。亮氨酰 - 、赖氨酰 - 、甲硫氨酰 - 、天冬氨酰 - 、组氨酰 - 和酪氨酰 - tRNA同功受体种类的相对比例存在显著差异。根据溴化氰与修饰核苷queuosine的反应标准，卡氏棘阿米巴天冬氨酰 - tRNA中queuosine的含量在成熟包囊中显著高于滋养体。天冬氨酰 - 、组氨酰 - 和酪氨酰 - tRNA反相5色谱洗脱图谱变化的相似性表明，一种共同机制导致了色谱图谱的改变。
