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Microanalytical RPC-5 columns for rapid chromatography of aminoacyl-tRNAs.

作者信息

Hernández-Velarde R, Hedgcoth C

出版信息

Arch Invest Med (Mex). 1978;9(4):519-26.

PMID:215093
Abstract

A microanalytical reversed-phase chromatographic system for the separation of aminoacyl-tRNA's on microbore (0.3cm X 25cm) columns is described. Chromatography of lysyl-tRNA from polyoma virus transformed mouse fibroblasts revealed at least seven identifiable isoacceptors. The system has the advantages of speed, sensitivity and excellent resolution of isoaccepting tRNA's.

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