Goullet P
C R Seances Acad Sci D. 1980 Jan 21;290(3):211-4.
Cellular esterases of 14 strains of Klebsiella pneumoniae, 14 strains of K. oxytoca, 16 strains of Enterobacter aerogenes and 16 strains of E. gergoviae were analysed by polyacrylamide-agarose gel and polyacrylamide gradient gel electrophoresis. Four principal esterase bands and nine minor bands, differing in their activity towards synthetic substrates and in their sensitivity to heat and to di-isofluoropropyl phosphate (DEP) were defined. The comparative distribution of bands showed that the four species analysed were characterized by distinct electrophoretic patterns of their esterases.
采用聚丙烯酰胺 - 琼脂糖凝胶电泳和聚丙烯酰胺梯度凝胶电泳对14株肺炎克雷伯菌、14株产酸克雷伯菌、16株产气肠杆菌和16株杰氏肠杆菌的细胞酯酶进行了分析。确定了四条主要酯酶带和九条次要酯酶带,它们对合成底物的活性、对热以及对二异氟丙基磷酸(DEP)的敏感性各不相同。条带的比较分布表明,所分析的这四个菌种具有各自独特的酯酶电泳图谱特征。