Loren A B, Horita M, Tseng C H, Yokoyama M M
Int Arch Allergy Appl Immunol. 1980;62(2):121-5. doi: 10.1159/000232503.
With the exception of the EA rosette test using SRBC, all other assay systems using BRBC and HRBC showed a significant dose response in rosette-forming cell count when the sensitizing antibody was further diluted. The percent of rosette-forming cell counts of the EA assay with SRBC, however, was unaffected by dilutions of antiserum even at exceedingly high levels. The present results suggest using red cells, which are nonreactive with T lumphocytes for the assay of B lymphocytes, and the use of antibody for sensitization of red blood cells must be adjusted for the optimal dilution necessary for proper EA rosette formation.
除了使用绵羊红细胞（SRBC）的E玫瑰花结试验外，当致敏抗体进一步稀释时，所有其他使用牛红细胞（BRBC）和人红细胞（HRBC）的检测系统在玫瑰花结形成细胞计数上均显示出显著的剂量反应。然而，即使在极高稀释度下，使用SRBC的E玫瑰花结试验中玫瑰花结形成细胞计数的百分比也不受抗血清稀释的影响。目前的结果表明，使用与T淋巴细胞无反应的红细胞来检测B淋巴细胞，并且必须调整用于红细胞致敏的抗体稀释度，以达到形成合适E玫瑰花结所需的最佳稀释度。
