Graham G G, Glover D V, Lopez de Romaña G, Morales E, MacLean W C
J Nutr. 1980 May;110(5):1061-9. doi: 10.1093/jn/110.5.1061.
The opaque-2 (o2) gene increases the lysine and tryptophan contents of maize and its protein quality; the sugary-2 (su2) gene improves vitreousness and density but decreases lysine; the double-mutant surgery-2 opaque-2 (su2o2) has the improved kernel characteristics and an even higher protein quality than opaque-2. Digestibility of energy and protein, and protein quality of normal, o2 and su2o2, both as endosperm (E) and whole kernel (WK) meals, were studied in eight convalescent malnourished children, 10--25 months of age. Diets provided 100--125 kcal/kg/day, 10% fat, with all of 6.4% protein calories from one of six maize meals or casein. Endosperm meals provided 83.2, 91.2 and 82.1% of energy, WK meals 73.2, 68.6 and 67.2% of energy in respective diets. Apparent N retention from E meals was lower than from WK meals, both lower than from casein. For each type of meal (E and WK) there was strong correlation between lysine "absorbed" and N retention, but this was higher from WK at equal lysine intakes. For 50% of children to match N retention from casein, presumably equal to requirement, they would have to consume 203.9, 148 or 122.5% of energy requirement as normal, o2 or su2o2 E meals, obviously impossible. For WK meals, they would have to consume 108.2, 90.3 or 84.2% of energy as normal, o2 or su2o2.
不透明-2(o2)基因可提高玉米的赖氨酸和色氨酸含量及其蛋白质品质;含糖-2(su2)基因可改善玉米的透明度和密度,但会降低赖氨酸含量;双突变体粉质-2不透明-2(su2o2)具有改良的籽粒特性,且蛋白质品质比不透明-2更高。对8名10至25个月大的营养不良恢复期儿童,研究了普通、o2和su2o2这三种类型的胚乳(E)和全籽粒(WK)粉的能量和蛋白质消化率以及蛋白质品质。饮食提供100 - 125千卡/千克/天,10%脂肪,其中6.4%的蛋白质热量全部来自六种玉米粉或酪蛋白中的一种。胚乳粉分别提供了各自饮食中83.2%、91.2%和82.1%的能量,全籽粒粉分别提供了73.2%、68.6%和67.2%的能量。胚乳粉的表观氮保留率低于全籽粒粉,两者均低于酪蛋白。对于每种类型的粉(胚乳和全籽粒),“吸收”的赖氨酸与氮保留之间存在强相关性,但在等量赖氨酸摄入量时,全籽粒粉的相关性更高。对于50%的儿童来说,要达到酪蛋白的氮保留量(大概等同于需求量),作为普通、o2或su2o2胚乳粉,他们需要消耗能量需求量的203.9%、148%或122.5%,这显然是不可能的。对于全籽粒粉,作为普通、o2或su2o2,他们需要消耗能量需求量的108.2%、90.3%或84.2%。