用于直接鉴定临床标本中立克次体的高滴度、群特异性荧光抗体试剂的研制与特性分析。
Development and characterization of high-titered, group-specific fluorescent-antibody reagents for direct identification of rickettsiae in clinical specimens.
作者信息
Hébert G A, Tzianabos T, Gamble W C, Chappell W A
出版信息
J Clin Microbiol. 1980 May;11(5):503-7. doi: 10.1128/jcm.11.5.503-507.1980.
Abstract
Rabbits were inoculated with purified antigen preparations of Coxiella burnetii and representative species of the spotted fever and typhus groups of rickettsiae. Their antibody responses were monitored by complement fixation tests; high-titered antisera were fractionated with ammonium sulfate and then labeled with fluorescein isothiocyanate by the dialysis method. The conjugates had homologous 3+ staining titers of 1:256 to 1:2,048 and did not exhibit nonspecific staining. The Rickettsia rickettsii, R. conorii, and R. akari conjugates reacted only with rickettsiae of the spotted fever group; the R. canada, R. prowazekii, and R. typhi conjugates were specific for the typhus group rickettsiae; and the C. burnetii conjugate stained only homologous organisms. One of these conjugates (R. rickettsii) is currently being used to identify rickettsiae in clinical specimens and has already proven its value as a diagnostic tool.
摘要
用纯化的伯氏考克斯体抗原制剂以及斑点热群和斑疹伤寒群立克次体的代表性菌种接种兔子。通过补体结合试验监测它们的抗体反应；高滴度抗血清用硫酸铵分级分离，然后通过透析法用异硫氰酸荧光素标记。这些结合物的同源3+染色滴度为1:256至1:2048，且未表现出非特异性染色。立氏立克次体、康氏立克次体和小蛛立克次体结合物仅与斑点热群立克次体反应；加拿大立克次体、普氏立克次体和伤寒立克次体结合物对斑疹伤寒群立克次体具有特异性；而伯氏考克斯体结合物仅对同源生物体染色。其中一种结合物（立氏立克次体）目前正用于鉴定临床标本中的立克次体，并且已经证明了其作为诊断工具的价值。
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Development and characterization of high-titered, group-specific fluorescent-antibody reagents for direct identification of rickettsiae in clinical specimens.用于直接鉴定临床标本中立克次体的高滴度、群特异性荧光抗体试剂的研制与特性分析。
J Clin Microbiol. 1980 May;11(5):503-7. doi: 10.1128/jcm.11.5.503-507.1980.
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