细菌直接荧光抗体试剂的最佳荧光素与蛋白质比例。
Optimal fluorescein-to-protein ratios of bacterial direct fluorescent-antibody reagents.
作者信息
Hébert G A, Pittman B, McKinney R M
出版信息
J Clin Microbiol. 1981 Mar;13(3):498-502. doi: 10.1128/jcm.13.3.498-502.1981.
Abstract
A number of bacterial systems were studied with specific direct fluorescent-antibody reagents prepared from rabbit antiserum fractions and having a wide range of fluorescein-to-protein ratios. These systems included Bacteroides, Bordetella, Clostridium, Escherichia, Legionella, Listeria, Salmonella, Shigella, and Streptococcus. For all systems studied, a fluorescein-to-protein ratio of 30 was optimal for conjugates prepared from ammonium sulfate fractions (greater than 75% gamma globulin) and pure immunoglobulin G desorbed from the Sepharose-bound protein A of Staphylococcus aureus. A pepsin digestion procedure is described that yielded the F(ab')2 piece of pure immunoglobulin G; this was labeled and studied at two fluorescein-to-protein ratios.
摘要
使用从兔抗血清组分制备的、具有广泛荧光素与蛋白质比例的特异性直接荧光抗体试剂，对多种细菌系统进行了研究。这些系统包括拟杆菌属、博德特氏菌属、梭菌属、大肠杆菌属、军团菌属、李斯特菌属、沙门氏菌属、志贺氏菌属和链球菌属。对于所有研究的系统，荧光素与蛋白质比例为30对于从硫酸铵组分（大于75%γ球蛋白）和从金黄色葡萄球菌的葡萄球菌A蛋白结合琼脂糖上解吸的纯免疫球蛋白G制备的结合物而言是最佳的。描述了一种胃蛋白酶消化程序，该程序产生了纯免疫球蛋白G的F(ab')2片段；对其进行了标记，并在两种荧光素与蛋白质比例下进行了研究。
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