Hunder G G, McDuffie F C, Clark R J
J Clin Lab Immunol. 1979 Nov;2(4):269-73.
Five sera and four synovial fluids from patients with rheumatoid arthritis were found to contain substances which consumed hemolytic C3 in normal human serum (NHS) and in normal guinea pig serum (NGPS). These fluids were then tested for ability to activate the alternative pathway by incubating them with NHS containing MgEGTA and C4 deficient GPS. All sera and two synovial fluids depleted C3 in these reagents, indicating direct activation by the alternative pathway. Density gradient ultracentrifugation demonstrated C3 fixing activity in some specimens in the greater than 19s regions. These substances may also activate complement similarly in vivo and participate in the development of inflammatory processes associated with this disease.
研究发现，类风湿性关节炎患者的5份血清和4份滑液中含有能消耗正常人血清（NHS）和正常豚鼠血清（NGPS）中溶血C3的物质。随后，通过将这些液体与含MgEGTA的NHS和C4缺陷型GPS孵育，测试它们激活替代途径的能力。所有血清和两份滑液均使这些试剂中的C3消耗殆尽，表明替代途径直接激活。密度梯度超速离心显示，部分标本在大于19s区域具有C3固定活性。这些物质在体内可能同样会激活补体，并参与与该疾病相关的炎症过程的发展。
