Fujitaki J M, Steiner A W, Nichols S E, Helander E R, Lin Y C, Smith R A
Prep Biochem. 1980;10(2):205-13. doi: 10.1080/00327488008061735.
An improved specific synthesis of N-epsilon-phospholysine is described. Phosphorylation under basic conditions of the copper chelate of L-lysine with phosphorus oxychloride and subsequent removal of the copper ion affords N-epsilon-phospholysine as the sole phosphorylated product. Similar treatment of L-arginine yields N-omega-phosphoarginine as the sole product which is identical to that produced enzymatically.
描述了一种改进的N-ε-磷酰赖氨酸的特异性合成方法。L-赖氨酸的铜螯合物在碱性条件下与三氯氧磷进行磷酸化反应，随后除去铜离子，得到N-ε-磷酰赖氨酸作为唯一的磷酸化产物。对L-精氨酸进行类似处理，得到N-ω-磷酰精氨酸作为唯一产物，该产物与酶促反应产生的产物相同。
