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[不同浓度的HCO₃⁻和二氧化碳对某些酶活性的影响]

[Effect of HCO3- and carbon dioxide at various concentrations on activity of certain enzymes].

作者信息

Mel'nikhuk D A, Siliunova N V, Shevchenko M I

出版信息

Ukr Biokhim Zh (1978). 1980 Mar-Apr;52(2):164-6.

PMID:6770515
Abstract

The paper deals with the effect of changes in the concentration of carbonic acid in the medium on the reaction rate catalyzed with enzymes of various spectrum of the action. It is shown that the presence of carbonic acid in the medium reaction increases the rate of reactions catalyzed with lactate dehydrogenase of the rabbit liver soluble fraction, with glucose-6-phosphate dehydrogenase from yeast and trypsin. Under the same conditions the reaction rate catalyzed with glucose-6-phosphate dehydrogenase of the rabbit liver soluble fraction and with ATP-citrate (pro-3S)-lyase is considerably decreased. Changes in the carbonic acid concentrations within the physiological limits are found to have no effect on lactate dehydrogenase from the cattle heart and chymotrypsin.

摘要

本文探讨了介质中碳酸浓度的变化对各种作用谱的酶催化反应速率的影响。结果表明,介质反应中碳酸的存在会提高兔肝可溶性部分的乳酸脱氢酶、酵母的葡萄糖-6-磷酸脱氢酶和胰蛋白酶所催化反应的速率。在相同条件下,兔肝可溶性部分的葡萄糖-6-磷酸脱氢酶和ATP-柠檬酸(pro-3S)裂合酶所催化的反应速率显著降低。发现在生理限度内碳酸浓度的变化对牛心乳酸脱氢酶和胰凝乳蛋白酶没有影响。

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