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高赖氨酸突变对大麦籽粒蛋白质组分的影响。

Effect of high-lysine mutations on the protein fractions of barley grain.

作者信息

Shewry P R, Faulks A J, Miflin B J

出版信息

Biochem Genet. 1980 Feb;18(1-2):133-51. doi: 10.1007/BF00504365.

DOI:10.1007/BF00504365
PMID:6770843
Abstract

We have studied the effects of six high-lysine barley mutations (Risø mutants 1508 and 56, Notch 1 and 2, lys 95 and 449) on the protein fractions of the grain. All mutants had a decreased relative and total amount of the lysine-poor hordein fraction, but only in Risø 1508 and 56 was the polypeptide composition of this fraction greatly affected. In all the mutants there were increases in the lysine-rich glutelin proteins and in nonprotein N while in Risø 1508 and the Notch mutants the total amount of salt-soluble proteins was also increased and their relative polypeptide composition substantially altered.

摘要

我们研究了六种高赖氨酸大麦突变体(里瑟突变体1508和56、缺口1和2、lys 95和449)对谷物蛋白质组分的影响。所有突变体中,赖氨酸含量低的醇溶蛋白组分的相对含量和总量均有所降低,但只有里瑟1508和56中该组分的多肽组成受到极大影响。在所有突变体中,富含赖氨酸的谷蛋白和非蛋白氮含量均增加,而在里瑟1508和缺口突变体中,盐溶性蛋白质总量也增加,其相对多肽组成发生了显著变化。

相似文献

1
Effect of high-lysine mutations on the protein fractions of barley grain.高赖氨酸突变对大麦籽粒蛋白质组分的影响。
Biochem Genet. 1980 Feb;18(1-2):133-51. doi: 10.1007/BF00504365.
2
Molecular analysis of a mutation conferring the high-lysine phenotype on the grain of barley (Hordeum vulgare).赋予大麦(Hordeum vulgare)籽粒高赖氨酸表型的突变的分子分析。
Cell. 1983 Aug;34(1):161-7. doi: 10.1016/0092-8674(83)90146-0.
3
Amino acid compositions of different protein fractions in developing grains of NP 113 barley and its high lysine Notch-2 mutant.NP 113大麦及其高赖氨酸Notch-2突变体发育籽粒中不同蛋白质组分的氨基酸组成
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4
Differential effects of high-lysine mutations on the accumulation of individual members of a group of proteins encoded by a disperse multigene family in the endosperm of barley (Hordeum vulgare L.).高赖氨酸突变对大麦(Hordeum vulgare L.)胚乳中由一个分散多基因家族编码的一组蛋白质的各个成员积累的差异影响。
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5
Molecular analysis of the effects of the lys 3a gene on the expression of Hor loci in developing endosperms of barley (Hordeum vulgare L.).赖氨酸3a基因对大麦(Hordeum vulgare L.)发育胚乳中Hor基因座表达影响的分子分析。
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Studies on the proteins of the mutants of barley grain. 3. Fractionation and characterization of the glutelin fraction.大麦籽粒突变体蛋白质的研究。3. 谷蛋白组分的分级分离与特性分析。
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The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.).胰蛋白酶抑制剂CMe的基因在大麦(Hordeum vulgare L.)胚乳中受lys 3a位点的反式调控。
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本文引用的文献

1
Second Mutant Gene Affecting the Amino Acid Pattern of Maize Endosperm Proteins.第二个影响玉米胚乳蛋白质氨基酸模式的突变基因。
Science. 1965 Dec 10;150(3702):1469-70. doi: 10.1126/science.150.3702.1469.
2
MUTANT GENE THAT CHANGES PROTEIN COMPOSITION AND INCREASES LYSINE CONTENT OF MAIZE ENDOSPERM.改变玉米胚乳蛋白质组成并增加赖氨酸含量的突变基因。
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3
Fractionation of hordein by preparative, continuous carrier-free electrophoresis.通过制备性连续无载体电泳对大麦醇溶蛋白进行分级分离。
可利用蛋氨酸浓度的提高与普通菜豆种子中高水平的伴球蛋白有关。
Theor Appl Genet. 1984 Nov;69(1):47-53. doi: 10.1007/BF00262537.
4
The polymorphism and structural homology of storage polypeptides (hordein) coded by the Hor-2 locus in barley (Hordeum vulgare L).大麦(Hordeum vulgare L.)中由Hor-2基因座编码的贮藏多肽(大麦醇溶蛋白)的多态性和结构同源性。
Biochem Genet. 1981 Oct;19(9-10):841-58. doi: 10.1007/BF00504250.
5
The ra locus and legumin synthesis in Pisum sativum.豌豆中ra基因座与豆球蛋白的合成
Biochem Genet. 1980 Dec;18(11-12):1207-19. doi: 10.1007/BF00484348.
6
Molecular analysis of the effects of the lys 3a gene on the expression of Hor loci in developing endosperms of barley (Hordeum vulgare L.).赖氨酸3a基因对大麦(Hordeum vulgare L.)发育胚乳中Hor基因座表达影响的分子分析。
Biochem Genet. 1984 Apr;22(3-4):231-55. doi: 10.1007/BF00484227.
7
The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.).胰蛋白酶抑制剂CMe的基因在大麦(Hordeum vulgare L.)胚乳中受lys 3a位点的反式调控。
Mol Gen Genet. 1989 Nov;219(3):474-9. doi: 10.1007/BF00259622.
8
Methylation of B-hordein genes in barley endosperm is inversely correlated with gene activity and affected by the regulatory gene Lys3.大麦胚乳中B-醇溶蛋白基因的甲基化与基因活性呈负相关,并受调控基因Lys3的影响。
Proc Natl Acad Sci U S A. 1992 May 1;89(9):4119-23. doi: 10.1073/pnas.89.9.4119.
Can J Biochem. 1968 Oct;46(10):1301-7. doi: 10.1139/o68-195.
4
Gene for improved nutritional value in barley seed protein.大麦种子蛋白中提高营养价值的基因。
Science. 1970 May 22;168(3934):985-7. doi: 10.1126/science.168.3934.985.
5
Studies on the proteins of the mutants of barley grain. 2. Fractionation and characterization of the alcohol-soluble proteins.大麦籽粒突变体的蛋白质研究。2. 醇溶蛋白的分级分离与特性分析。
J Agric Food Chem. 1977 Jul-Aug;25(4):912-7. doi: 10.1021/jf60212a048.