Davies D R
Biochem Genet. 1980 Dec;18(11-12):1207-19. doi: 10.1007/BF00484348.
Cellulose acetate electrophoresis has been used to resolve the storage proteins of peas into their constituent groups. Comparisons of 171 randomly chosen genotypes representing primitive forms, subspecies, and cultivars of peas, of seven near-isogenic lines for found and wrinkled and of two F2 populations have shown that wrinkled seed has a lower proportion of legumin than round seed. The extent of the reduction varies with the background genotype; some of the wrinkled forms has less than one-third as much legumin as their isogenic round forms. This effect of the ra locus on storage protein composition provides the first example in peas of a mutant analogous to the op 2 and fl 2 mutants in maize. Sodium dodecyl sulphate polyacrylamide gel electrophoresis was used to discriminate the 40 kdalton (alpha subunits) of legumin. On the basis of the data obtained from F2 populations derived from genotypes with distinct alpha subunit patterns, it was shown that the structural genes for the alpha subunit polypeptides of legumin are on chromosome 7, and closely linked to the ra locus.
醋酸纤维素电泳已被用于将豌豆的贮藏蛋白分离成其组成组。对代表豌豆原始类型、亚种和栽培品种的171个随机选择的基因型、7个圆粒和皱粒近等基因系以及2个F2群体的比较表明，皱粒种子中豆球蛋白的比例低于圆粒种子。减少的程度因背景基因型而异；一些皱粒类型的豆球蛋白含量不到其同基因圆粒类型的三分之一。ra基因座对贮藏蛋白组成的这种影响为豌豆中第一个类似于玉米op 2和fl 2突变体的突变体提供了例子。十二烷基硫酸钠聚丙烯酰胺凝胶电泳用于区分豆球蛋白的40千道尔顿（α亚基）。根据从具有不同α亚基模式的基因型衍生的F2群体获得的数据，表明豆球蛋白α亚基多肽的结构基因位于7号染色体上，并且与ra基因座紧密连锁。
