Chooi W Y, Sabatini L M, Macklin M, Fraser D
Biochemistry. 1980 Apr 1;19(7):1425-33. doi: 10.1021/bi00548a025.
Proteins were extracted from ribosomes and (for the first time) from ribosomal subunits of Drosophila melanogaster embryos. The ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. The electrophoretograms displayed 78 spots for the 80S monomers, 35 spots for the 60S subunits, and 31 spots for the 40S subunits. On the basis of present information, we propose what we believe to be a reliable and convenient nomenclature for the proteins of the ribosomes and each of the subunits. A pair of acidic proteins from D. melanogaster appears to be very similar in electrophoretic mobility to the acidic proteins L7/L12 from Escherichia coli and L40/L41 from rat liver. The electrophoretogram of proteins from embryonic ribosomes shows both qualitative and quantitative differences from those of larvae, pupae, and adults previously reported by others. The proteins of the 40S subunit range in molecular weight from approximately 10,000 to 50,000, and those from the 60S subunit range from approximately 11,000 to 50,000.
蛋白质从果蝇胚胎的核糖体中提取出来（首次），也从核糖体亚基中提取出来。核糖体蛋白质通过二维聚丙烯酰胺凝胶电泳进行分析。电泳图谱显示，80S单体有78个斑点，60S亚基有35个斑点，40S亚基有31个斑点。根据目前的信息，我们提出了一种我们认为可靠且方便的核糖体及每个亚基蛋白质的命名法。一对来自黑腹果蝇的酸性蛋白质在电泳迁移率上似乎与来自大肠杆菌的酸性蛋白质L7/L12以及来自大鼠肝脏的酸性蛋白质L40/L41非常相似。胚胎核糖体蛋白质的电泳图谱与其他人之前报道的幼虫、蛹和成虫的电泳图谱在定性和定量上都存在差异。40S亚基的蛋白质分子量范围约为10,000至50,000，60S亚基的蛋白质分子量范围约为11,000至50,000。
