Chooi W Y, Sabatini L M, Macklin M
Biochem Genet. 1984 Aug;22(7-8):749-67. doi: 10.1007/BF00485858.
Antibodies raised against Drosophila melanogaster ribosomal proteins (r-proteins) were used to examine possible structural relationships between eukaryotic and prokaryotic r-proteins. The antisera were raised against either groups of r-proteins or individually purified r-proteins. Two antisera showed a cross-reaction with total Escherichia coli r-proteins in Ouchterlony double immunodiffusion assays: an antiserum against the D. melanogaster small subunit protein S14 (anti-S14) and an antiserum against a group of D. melanogaster r-proteins (anti-TP80). The specificity of the antisera and the identity of the homologous E. coli r-proteins were characterized by using immunooverlay and immunoblot assays. These assays indicated that anti-S14 was highly specific for protein S14 and anti-TP80 was a multispecific serum that recognized several of the D. melanogaster ribosomal proteins. The E. coli protein homologous to D. melanogaster protein S14 was identified as E. coli protein S6. By adsorption of the anti-TP80 serum, we determined that D. melanogaster protein 7/8 is homologous to the acidic E. coli protein L7/L12. D. melanogaster acidic protein 13 was also shown to be immunologically related to D. melanogaster protein 7/8.
利用针对黑腹果蝇核糖体蛋白（r - 蛋白）产生的抗体来研究真核生物和原核生物r - 蛋白之间可能的结构关系。抗血清是针对r - 蛋白组或单独纯化的r - 蛋白产生的。在双向免疫扩散试验中，两种抗血清与大肠杆菌总r - 蛋白发生了交叉反应：一种是针对黑腹果蝇小亚基蛋白S14的抗血清（抗 - S14），另一种是针对一组黑腹果蝇r - 蛋白的抗血清（抗 - TP80）。通过免疫覆盖和免疫印迹分析对抗血清的特异性和同源大肠杆菌r - 蛋白的身份进行了表征。这些分析表明，抗 - S14对蛋白S14具有高度特异性，而抗 - TP80是一种多特异性血清，可识别几种黑腹果蝇核糖体蛋白。与黑腹果蝇蛋白S14同源的大肠杆菌蛋白被鉴定为大肠杆菌蛋白S6。通过吸附抗 - TP80血清，我们确定黑腹果蝇蛋白7/8与酸性大肠杆菌蛋白L7/L12同源。黑腹果蝇酸性蛋白13也被证明与黑腹果蝇蛋白7/8存在免疫相关性。
