通过二维薄层色谱法分离氨基糖及相关化合物。
Separation of amino sugars and related compounds by two-dimensional thin-layer chromatography.
作者信息
Ryan E A, Kropinski A M
出版信息
J Chromatogr. 1980 Jul 4;195(1):127-32. doi: 10.1016/s0021-9673(00)81550-2.
PMID:6771303
Abstract
Separation of amino compounds, including galactosamine and glucosamine, found in lipopolysaccharide and peptidoglycan, can be accomplished by two-dimensional thin-layer chromatography on cellulose layers. The solvent systems used are: 2-propanol-90% formic acid-water (80:4:20,v/v) in the first dimension and lutidine-water (65:35, v/v) in the second dimension. Compounds were detected using a chromogenic ninhydrin spray.
摘要
存在于脂多糖和肽聚糖中的氨基化合物（包括半乳糖胺和葡萄糖胺）的分离，可以通过在纤维素层上进行二维薄层色谱法来完成。使用的溶剂系统为：第一维是2-丙醇-90%甲酸-水（80:4:20，v/v），第二维是二甲基吡啶-水（65:35，v/v）。使用显色茚三酮喷雾剂检测化合物。
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