Völkl A, Berlet H H
J Clin Chem Clin Biochem. 1977 May;15(5):267-9. doi: 10.1515/cclm.1977.15.1-12.267.
Twelve guanidino compounds including, among others, glycocyamine, creatine, creatinine, arginine, guanidinosuccinic acid were submitted to thin-layer chromatography on plates precoated with silica gel or cellulose, respectively. Optimal resolution was obtained by two-dimensional chromatography on cellulose layers when the two solvent systems iso-propanol-acetic acid-water (volumes, 60 ml + 45 ml + 15 ml) and iso-propanol-dimethyl-formamide-water (volumes, 40 ml + 20 ml + 20 ml) were used for the first and second dimension, respectively. This procedure permitted the resolution of 10 distinct spots out of a mixture of the twelve guanidino compounds. Chloroform-methanol-ammonia (170 g/kg) (volumes, 40 ml + 20 ml + 20 ml) proved to be the most suitable solvent system for one-dimensional chromatography on cellulose layers of less complex mixtures of guanidino compounds.
十二种胍基化合物，其中包括胍基乙胺、肌酸、肌酐、精氨酸、胍基琥珀酸等，分别在预涂硅胶或纤维素的薄板上进行薄层色谱分析。当分别使用异丙醇-乙酸-水（体积比为60 ml + 45 ml + 15 ml）和异丙醇-二甲基甲酰胺-水（体积比为40 ml + 20 ml + 20 ml）这两种溶剂系统在纤维素层上进行二维色谱分析时，可获得最佳分离效果。该方法能够从十二种胍基化合物的混合物中分离出10个清晰的斑点。对于胍基化合物不太复杂的混合物在纤维素层上进行一维色谱分析时，氯仿-甲醇-氨（170 g/kg）（体积比为40 ml + 20 ml + 20 ml）被证明是最合适的溶剂系统。
