盐酸8-（N，N-二乙氨基）辛酯-3,4,5-三甲氧基苯甲酸酯（TMB-8）对蜡样芽孢杆菌T孢子萌发的抑制作用。

Inhibitory effect of 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8) on germination of Bacillus cereus T spores.

作者信息

Shibata H, Mimura A, Mitani H, Tani I, Hashimoto T

机构信息

Department of Microbial Chemistry, Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.

出版信息

Microbiol Immunol. 1993;37(1):63-7. doi: 10.1111/j.1348-0421.1993.tb03180.x.

DOI:10.1111/j.1348-0421.1993.tb03180.x

PMID:8474359

Abstract

Effect of 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8), a calcium antagonist, on germination of Bacillus cereus T spores induced by L-alanine and inosine was investigated. TMB-8 had no effect on the germination of heat-activated spores, whereas it inhibited that of nonactivated spores. The TMB-8 inhibitory effect was antagonized competitively by inosine, but not by L-alanine. Addition of Ca2+ reversed the inhibitory effect of TMB-8 in a dose-related fashion. Based on the results, a role of inosine and a site(s) for inhibitory action of TMB-8 in the process leading to the germination of nonactivated spores were discussed.

摘要

研究了钙拮抗剂盐酸8 -（N，N - 二乙氨基）辛酯 - 3,4,5 - 三甲氧基苯甲酸酯（TMB - 8）对L - 丙氨酸和肌苷诱导的蜡样芽孢杆菌T孢子萌发的影响。TMB - 8对热激活孢子的萌发没有影响，而抑制未激活孢子的萌发。肌苷可竞争性拮抗TMB - 8的抑制作用，而L - 丙氨酸则不能。添加Ca2 +可剂量依赖性地逆转TMB - 8的抑制作用。基于这些结果，讨论了肌苷的作用以及TMB - 8在未激活孢子萌发过程中的抑制作用位点。