绿蟾蜍（Bufo viridis）体内的酸碱状态

Acid-base status in the toad Bufo viridis in vivo.

作者信息

Katz U

出版信息

Respir Physiol. 1980 Jul;41(1):105-15. doi: 10.1016/0034-5687(80)90026-2.

Abstract

The acid-base status in the toad Bufo viridis was assessed on blood samples taken through a chronical cannula from unrestrained animals. Blood gases and the pH were very constant in animals which were kept either under control conditions (free access to tap water) or immersed in shallow water. pH was 7.646 +/- 0.032 (mean +/- SEM) in 14 control toads at 26 degrees C. PCO2 was 13.1 +/- 1.2 mm Hg and [HCO3-] was 17.0 +/- 0.7 mmol/l at the same temperature. pH and PCO2 were independent of the hematocrit and the haemoglobin in the blood. Diamox induced a characteristic metabolic acidosis which is apprently the result of the inhibition of carbonic anhydrase both in the kidney and in the skin. The results are discussed in relation to the role of the kidney and the skin in regulating the acid-base balance in the toad.

摘要

通过长期插管从自由活动的动物身上采集血样，评估绿蟾蜍的酸碱状态。在处于对照条件（可自由获取自来水）或浸泡在浅水中的动物中，血气和pH值非常稳定。在26摄氏度下，14只对照蟾蜍的pH值为7.646±0.032（平均值±标准误）。在相同温度下，PCO2为13.1±1.2 mmHg，[HCO3-]为17.0±0.7 mmol/L。pH值和PCO2与血液中的血细胞比容和血红蛋白无关。乙酰唑胺诱发了一种典型的代谢性酸中毒，这显然是肾脏和皮肤中碳酸酐酶受到抑制的结果。结合肾脏和皮肤在调节蟾蜍酸碱平衡中的作用对结果进行了讨论。