通过相应的糖基甲基对硝基苯基三氮烯对活成纤维细胞中溶酶体糖苷酶进行特异性失活。
Specific inactivation of lysosomal glycosidases in living fibroblasts by the corresponding glycosylmethyl-p-nitrophenyltriazenes.
作者信息
Van Diggelen O P, Galjaard H, Sinnott M L, Smith P J
出版信息
Biochem J. 1980 May 15;188(2):337-43. doi: 10.1042/bj1880337.
Abstract
- beta-D-Galactopyranosylmethyl-p-nitrophenyltriazene can specificag detectable changes in cell viability or the activities of other hydrolases. 2. beta-D-Glucopyranosylmethyl-p-nitrophenyltriazene behaves similarly towards lysosomal beta-glucosidase, but also inactivates some alpha-glucosidase. 3. Both beta-galactosidase and beta-glucosidase activities in triazene-treated confluent fibroblast cultures recover exponentially; if zero-order enzyme production is assumed, turnover times of 10 and 5 days respectively can be estimated.
摘要
- β-D-吡喃半乳糖基甲基对硝基苯三氮烯能特异性地检测到细胞活力或其他水解酶活性的变化。2. β-D-吡喃葡萄糖基甲基对硝基苯三氮烯对溶酶体β-葡萄糖苷酶表现出类似的作用，但也会使一些α-葡萄糖苷酶失活。3. 在三氮烯处理的汇合成纤维细胞培养物中，β-半乳糖苷酶和β-葡萄糖苷酶的活性均呈指数恢复；如果假设酶的产生为零级反应，则可分别估计出周转时间为10天和5天。
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