通过相应的糖基甲基对硝基苯基三氮烯对活成纤维细胞中溶酶体糖苷酶进行特异性失活。
Specific inactivation of lysosomal glycosidases in living fibroblasts by the corresponding glycosylmethyl-p-nitrophenyltriazenes.
作者信息
Van Diggelen O P, Galjaard H, Sinnott M L, Smith P J
出版信息
Biochem J. 1980 May 15;188(2):337-43. doi: 10.1042/bj1880337.
Abstract
- beta-D-Galactopyranosylmethyl-p-nitrophenyltriazene can specificag detectable changes in cell viability or the activities of other hydrolases. 2. beta-D-Glucopyranosylmethyl-p-nitrophenyltriazene behaves similarly towards lysosomal beta-glucosidase, but also inactivates some alpha-glucosidase. 3. Both beta-galactosidase and beta-glucosidase activities in triazene-treated confluent fibroblast cultures recover exponentially; if zero-order enzyme production is assumed, turnover times of 10 and 5 days respectively can be estimated.
摘要
- β-D-吡喃半乳糖基甲基对硝基苯三氮烯能特异性地检测到细胞活力或其他水解酶活性的变化。2. β-D-吡喃葡萄糖基甲基对硝基苯三氮烯对溶酶体β-葡萄糖苷酶表现出类似的作用，但也会使一些α-葡萄糖苷酶失活。3. 在三氮烯处理的汇合成纤维细胞培养物中，β-半乳糖苷酶和β-葡萄糖苷酶的活性均呈指数恢复；如果假设酶的产生为零级反应，则可分别估计出周转时间为10天和5天。
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本文引用的文献
1
The kinetics of catalase synthesis and destruction in vivo.过氧化氢酶在体内合成与分解的动力学。
J Biol Chem. 1962 Nov;237:3468-75.
2
Isolation of beta-galactosidase and beta-glucosidase from brain.从大脑中分离β-半乳糖苷酶和β-葡萄糖苷酶。
Biochim Biophys Acta. 1966 Mar 7;113(3):567-76. doi: 10.1016/s0926-6593(66)80014-0.
3
Identity of beta-glucosidase, beta-xylosidase and one of the beta-galactosidase activities in human liver when assayed with 4-methylumbelliferyl-beta-D-glycosides studies in cases of Gaucher's disease.用4-甲基伞形酮基-β-D-糖苷测定时，人肝脏中β-葡萄糖苷酶、β-木糖苷酶及其中一种β-半乳糖苷酶活性的鉴定：戈谢病病例研究
Biochim Biophys Acta. 1968 Aug 6;165(1):59-62.
4
GM1 ganglioside beta-galactosidase. A. Purification and studies of the enzyme from human liver.GM1神经节苷脂β-半乳糖苷酶。A. 人肝脏中该酶的纯化及研究。
J Biol Chem. 1974 Dec 25;249(24):7969-76.
5
The turnover of hamster fibroblast lysosomal beta-D-glucuronidase.仓鼠成纤维细胞溶酶体β-D-葡萄糖醛酸酶的周转
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6
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Biochem J. 1976 Aug 15;158(2):401-7. doi: 10.1042/bj1580401.
7
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8
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9
Multiple carbohydrate-cleaving specificities in human acidic and neutral glycosidases.人类酸性和中性糖苷酶中的多种碳水化合物切割特异性。
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10
Sialidosis: a review of human neuraminidase deficiency.唾液酸沉积症：人类神经氨酸酶缺乏症综述
Am J Hum Genet. 1979 Jan;31(1):1-18.
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