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仓鼠成纤维细胞溶酶体β-D-葡萄糖醛酸酶的周转

The turnover of hamster fibroblast lysosomal beta-D-glucuronidase.

作者信息

Warburton M J, Wynn C H

出版信息

Biochem J. 1977 Jan 15;162(1):201-3. doi: 10.1042/bj1620201.

DOI:10.1042/bj1620201
PMID:849277
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164585/
Abstract

The half-life of hamster fibroblast beta-D-glucuronidase (EC 3.2.1.31) was estimated to be 4-5 days by measuring the decay with time of the radioactivity in beta-D-glucuronidase isolated from cells grown in the presence of 14C-labelled amino acids. A new affinity-chromatographic procedure for the purification of beta-D-glucuronidase is described.

摘要

通过测量在含有¹⁴C标记氨基酸的条件下培养的细胞中分离出的β-D-葡萄糖醛酸酶的放射性随时间的衰减，估计仓鼠成纤维细胞β-D-葡萄糖醛酸酶（EC 3.2.1.31）的半衰期为4至5天。本文描述了一种用于纯化β-D-葡萄糖醛酸酶的新的亲和色谱方法。

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1
The turnover of hamster fibroblast lysosomal beta-D-glucuronidase.仓鼠成纤维细胞溶酶体β-D-葡萄糖醛酸酶的周转
Biochem J. 1977 Jan 15;162(1):201-3. doi: 10.1042/bj1620201.
2
The effect of intralysosomal sucrose storage on the turnover of hamster fibroblast lysosomal and Golgi-apparatus enzymes.溶酶体内蔗糖储存对仓鼠成纤维细胞溶酶体和高尔基体酶周转的影响。
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A large-scale purification of beta-glucuronidase from human liver by immunoaffinity chromatography.通过免疫亲和色谱法从人肝脏中大规模纯化β-葡萄糖醛酸酶。
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引用本文的文献

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Biochem J. 1980 May 15;188(2):337-43. doi: 10.1042/bj1880337.
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Heterogeneity of lysosomal enzymes in cultured normal and sialidosis type II human fibroblasts and the effect of ammonium chloride on this heterogeneity.培养的正常人和II型唾液酸沉积症患者人成纤维细胞中溶酶体酶的异质性以及氯化铵对这种异质性的影响。
Mol Cell Biochem. 1985 May;67(1):25-30. doi: 10.1007/BF00220982.
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Purification and immunological quantification of rat liver lysosomal glycosidases.大鼠肝脏溶酶体糖苷酶的纯化及免疫定量分析
Biochem J. 1989 Nov 15;264(1):115-23. doi: 10.1042/bj2640115.
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Turnover of kidney beta-glucuronidase in normal and Chédiak-Higashi (beige) mice.正常小鼠和切-东（米色）小鼠肾脏β-葡萄糖醛酸酶的周转率
Am J Pathol. 1978 Sep;92(3):755-72.
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Evidence for lysosomal enzyme recognition by human fibroblasts via a phosphorylated carbohydrate moiety.人类成纤维细胞通过磷酸化碳水化合物部分识别溶酶体酶的证据。
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Acid hydrolase activity in red and white skeletal muscle of mice during a two-week period following exhausting exercise.
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本文引用的文献

1
The preparation and properties of beta-glucuronidase. IV. Inhibition by sugar acids and their lactones.β-葡萄糖醛酸酶的制备及其性质。IV. 糖酸及其内酯的抑制作用。
Biochem J. 1952 Nov;52(3):464-72. doi: 10.1042/bj0520464.
2
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
J Biol Chem. 1970 Jun;245(12):3059-65.
3
Synthesis and turnover of lysosomal glycoproteins. Relation to the molecular heterogeneity of the lysosomal enzymes.溶酶体糖蛋白的合成与周转。与溶酶体酶分子异质性的关系。
FEBS Lett. 1974 Feb 15;39(2):176-81. doi: 10.1016/0014-5793(74)80045-1.
4
Purification of beta-glucosidase activities from bovine spleen by affinity chromatography.通过亲和层析法从牛脾脏中纯化β-葡萄糖苷酶活性。
Anal Biochem. 1974 Jul;60(1):200-5. doi: 10.1016/0003-2697(74)90145-6.
5
Purification of alpha- and beta-galactosidases by affinity chromatography.通过亲和色谱法纯化α-半乳糖苷酶和β-半乳糖苷酶。
Anal Biochem. 1973 Sep;55(1):301-5. doi: 10.1016/0003-2697(73)90319-9.
6
Intracellular protein degradation in mammalian and bacterial cells.哺乳动物细胞和细菌细胞中的细胞内蛋白质降解
Annu Rev Biochem. 1974;43(0):835-69. doi: 10.1146/annurev.bi.43.070174.004155.
7
Relationship between digradation rates of proteins in vivo and their susceptibility to lysosomal proteases.体内蛋白质降解速率与其对溶酶体蛋白酶敏感性之间的关系。
J Biol Chem. 1974 Oct 10;249(19):6364-5.
8
The hyperactivity of hamster fibroblast lysosomal enzymes after endocytosis of sucrose.仓鼠成纤维细胞在摄取蔗糖后溶酶体酶的活性增强。
Biochem Biophys Res Commun. 1976 May 3;70(1):94-100. doi: 10.1016/0006-291x(76)91113-x.
9
Concerning a possible mechanism for selective capture of cytoplasmic proteins by lysosomes.关于溶酶体选择性捕获细胞质蛋白的可能机制。
Biochem Biophys Res Commun. 1975 Nov 17;67(2):604-9. doi: 10.1016/0006-291x(75)90855-4.
10
Turnover studies on proteins of rat liver lysosomes.大鼠肝脏溶酶体蛋白质的周转研究。
J Biol Chem. 1975 Jul 10;250(13):4896-902.