仓鼠成纤维细胞溶酶体β-D-葡萄糖醛酸酶的周转
The turnover of hamster fibroblast lysosomal beta-D-glucuronidase.
作者信息
Warburton M J, Wynn C H
出版信息
Biochem J. 1977 Jan 15;162(1):201-3. doi: 10.1042/bj1620201.
Abstract
The half-life of hamster fibroblast beta-D-glucuronidase (EC 3.2.1.31) was estimated to be 4-5 days by measuring the decay with time of the radioactivity in beta-D-glucuronidase isolated from cells grown in the presence of 14C-labelled amino acids. A new affinity-chromatographic procedure for the purification of beta-D-glucuronidase is described.
摘要
通过测量在含有¹⁴C标记氨基酸的条件下培养的细胞中分离出的β-D-葡萄糖醛酸酶的放射性随时间的衰减，估计仓鼠成纤维细胞β-D-葡萄糖醛酸酶（EC 3.2.1.31）的半衰期为4至5天。本文描述了一种用于纯化β-D-葡萄糖醛酸酶的新的亲和色谱方法。
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本文引用的文献
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The preparation and properties of beta-glucuronidase. IV. Inhibition by sugar acids and their lactones.β-葡萄糖醛酸酶的制备及其性质。IV. 糖酸及其内酯的抑制作用。
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