Lefevre M, Rucker R B
Biochim Biophys Acta. 1980 Jul 15;630(4):519-29. doi: 10.1016/0304-4165(80)90006-9.
The turnover and degradation of mature elastin from the aortae of Japanese quail were estimated following injection with L-[U-14C]lysine by measuring the changes in specific activity of L-[U-14C]lysine and 14C-labelled desmosine and isodesmosine (crosslinking amino acids derived from lysyl residues) in elastin over a 39-week period. Only 5% of the variation in radioactivity could be attributed to changes in time. Therefore, it was concluded that the best estimates of mature elastin turnover are only quantifiable in years. Dietary cholesterol in amounts sufficient to induce plaque formatioin and fragmentation of the elastic lamina in the aorta did not significantly influence turnover time. It would appear that once the total pool of elastin in aorta is stabilized as mature fibers it is not subject to proteolysis or resynthesis of sufficient magnitude to result in measurable turnover.
在给日本鹌鹑注射L-[U-¹⁴C]赖氨酸后，通过测量39周内弹性蛋白中L-[U-¹⁴C]赖氨酸以及¹⁴C标记的锁链素和异锁链素（源自赖氨酰残基的交联氨基酸）的比活性变化，来估计日本鹌鹑主动脉中成熟弹性蛋白的周转率和降解情况。放射性的变化中只有5%可归因于时间变化。因此，得出的结论是，成熟弹性蛋白周转率的最佳估计值仅能以年为单位进行量化。足以诱导主动脉中斑块形成和弹性层破碎的膳食胆固醇量并未显著影响周转时间。看起来，一旦主动脉中的弹性蛋白总量稳定为成熟纤维，它就不会受到足以导致可测量周转率的蛋白水解或再合成的影响。
