Deumié M, Bartholomew J C, Wade C G
C R Seances Acad Sci D. 1980 Jun 16;290(22):1417-20.
It is shown that in living cells, some liposoluble collisional quenchers like n-iodo hexane and iodo benzene can be used to selectively quench the benzo(a)pyrene fluorescence, leving the diol-epoxide benzo(a)pyrene metabolite emission. The preferential solubilities of these compounds, fluorophors and quenchers, explain the results and the fluorophor location.
结果表明,在活细胞中,一些脂溶性碰撞猝灭剂如正碘己烷和碘苯可用于选择性猝灭苯并(a)芘的荧光,而保留二醇环氧化物苯并(a)芘代谢物的发射。这些化合物、荧光团和猝灭剂的优先溶解性解释了实验结果和荧光团的位置。