Rylatt D B, Aitken A, Bilham T, Condon G D, Embi N, Cohen P
Eur J Biochem. 1980 Jun;107(2):529-37.
Glycogen synthase kinase-3 phosphorylates three serine residues on glycogen synthase (sites 3a, 3b and 3c) which are all located in the same nine-amino-acid segment of the polypeptide chain. The sequence in this region is: Arg-Tyr-Pro-Arg-Pro-Ala-Ser(P)-Val-Pro-Pro-Ser(P)-Pro-Ser-Leu-Ser(P)-Arg-. These serine residues are distinct from the sites phosphorylated preferentially by cyclic-AMP-dependent protein kinase (sites 1a and 1b) and phosphorylase kinase (site 2). The N-terminal sequence of glycogen synthase containing the serine residue phosphorylated by phosphorylase kinase has been extended. The sequence in this region is: Pro-Leu-Ser-Arg-Thr-Leu-Ser(P)-Val-Ser-Ser-Leu-Pro-Gly-Leu-Glu-Asp-Trp-Glu-Asp- Glu-Phe-Asp-Leu-Glu-Asn-Ser-Val-Leu-Phe-(Asx2,Glx2,Ala2,Val2,Lys)-. The similarity to the N-terminal sequence of phosphorylase is confined to the immediate vicinity of the phosphorylation site (residues 4--15). The relationship of glycogen synthase kinase-3 to glycogen synthase kinases that have been described by other laboratories is discussed.
糖原合酶激酶-3使糖原合酶上的三个丝氨酸残基（位点3a、3b和3c）发生磷酸化，这些残基均位于多肽链的同一个九氨基酸片段中。该区域的序列为：精氨酸-酪氨酸-脯氨酸-精氨酸-脯氨酸-丙氨酸-丝氨酸（磷酸化）-缬氨酸-脯氨酸-脯氨酸-丝氨酸（磷酸化）-脯氨酸-丝氨酸-亮氨酸-丝氨酸（磷酸化）-精氨酸-。这些丝氨酸残基不同于环磷酸腺苷依赖性蛋白激酶优先磷酸化的位点（位点1a和1b）以及磷酸化酶激酶的磷酸化位点（位点2）。含有被磷酸化酶激酶磷酸化的丝氨酸残基的糖原合酶N端序列已得到延伸。该区域的序列为：脯氨酸-亮氨酸-丝氨酸-精氨酸-苏氨酸-亮氨酸-丝氨酸（磷酸化）-缬氨酸-丝氨酸-丝氨酸-亮氨酸-脯氨酸-甘氨酸-亮氨酸-谷氨酸-天冬氨酸-色氨酸-谷氨酸-天冬氨酸-谷氨酸-苯丙氨酸-天冬氨酸-亮氨酸-谷氨酸-天冬酰胺-丝氨酸-缬氨酸-亮氨酸-苯丙氨酸-（天冬氨酸2、谷氨酸2、丙氨酸2、缬氨酸2、赖氨酸）-。与磷酸化酶N端序列的相似性仅限于磷酸化位点的紧邻区域（第4至15位残基）。文中还讨论了糖原合酶激酶-3与其他实验室所描述的糖原合酶激酶之间的关系。
