糖原合酶激酶3β（GSK3β）调控绵羊骨骼肌卫星细胞的成肌分化

Glycogen Synthase Kinase 3β (GSK3β) Regulates Myogenic Differentiation in Skeletal Muscle Satellite Cells of Sheep.

作者信息

Yang Jingquan, Yang Haosen, Wang Linjie, Zhou Ping

机构信息

State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

出版信息

Animals (Basel). 2022 Oct 15;12(20):2789. doi: 10.3390/ani12202789.

DOI:10.3390/ani12202789

PMID:36290175

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9597728/

Abstract

Glycogen synthase kinase 3β (GSK3β) has a vital role in the regulation of many cellular processes. However, the role of GSK3β in muscle cell differentiation in sheep remains unknown. In this study, we investigated the function of GSK3β in skeletal muscle satellite cells (SMSCs) of sheep. An overexpression of significantly inhibited myotube formation as well as the mRNA levels of myogenic genes (, , , and ) in sheep SMSCs. SB216763 treatment had a time-course effect on the phosphorylation levels of sheep GSK3β. In addition, reducing the activity of GSK3β lead to the promotion of sheep SMSCs differentiation as well as the mRNA levels of myogenic genes (, , , and ). This study illustrated the function of GSK3β to inhibit myogenesis in sheep SMSCs, which provided evidence for studying the mechanisms involved in the regulation of sheep SMSCs differentiation by GSK3β.

摘要

糖原合酶激酶3β（GSK3β）在许多细胞过程的调节中起着至关重要的作用。然而，GSK3β在绵羊肌肉细胞分化中的作用仍不清楚。在本研究中，我们研究了GSK3β在绵羊骨骼肌卫星细胞（SMSCs）中的功能。过表达显著抑制了绵羊SMSCs中的肌管形成以及成肌基因（、、、和）的mRNA水平。SB216763处理对绵羊GSK3β的磷酸化水平具有时间进程效应。此外，降低GSK3β的活性导致绵羊SMSCs分化以及成肌基因（、、、和）的mRNA水平的促进。本研究阐明了GSK3β在绵羊SMSCs中抑制肌生成的功能，为研究GSK3β调节绵羊SMSCs分化的机制提供了证据。