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猪源Fab μ片段和Fab α片段与金黄色葡萄球菌蛋白A的相互作用

Interactions of pig Fab mu and Fab alpha fragments with protein A from Staphylococcus aureus.

作者信息

Zikán J

出版信息

Folia Microbiol (Praha). 1980;25(3):254-8. doi: 10.1007/BF02877347.

DOI:10.1007/BF02877347
PMID:6772532
Abstract

Forty % of serum IgM and 47% of dimeric colostral IgA were bound to protein A-Sepharose. Fab mu and Fab alpha fragments showed a reactivity similar to that of the whole immunoglobulins. Complete elutions of IgM and IgA from the protein A-Sepharose were obtained in lower cocentrations of MgCl2 than the complete elution of IgG. However, IgM and IgA were completely eluted at higher concentrations of MgCl2 in the presence of IgG than in its absence.

摘要

40%的血清IgM和47%的二聚体初乳IgA与蛋白A-琼脂糖结合。Fab μ和Fab α片段表现出与完整免疫球蛋白相似的反应性。从蛋白A-琼脂糖上完全洗脱IgM和IgA所需的MgCl2浓度低于完全洗脱IgG所需的浓度。然而，在有IgG存在时，与无IgG时相比，在更高浓度的MgCl2下，IgM和IgA能被完全洗脱。

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1
Interactions of pig Fab mu and Fab alpha fragments with protein A from Staphylococcus aureus.猪源Fab μ片段和Fab α片段与金黄色葡萄球菌蛋白A的相互作用
Folia Microbiol (Praha). 1980;25(3):254-8. doi: 10.1007/BF02877347.
2
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Alternative mechanism of protein A-immunoglobulin interaction the VH-associated reactivity of a monoclonal human IgM.蛋白A与免疫球蛋白相互作用的另一种机制：单克隆人IgM的VH相关反应性
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引用本文的文献

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Cross-reactivity of human and a putative pig IgD. Pig IgD-like molecules as serum and lymphocyte components.人和一种假定的猪IgD的交叉反应性。猪IgD样分子作为血清和淋巴细胞成分。
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本文引用的文献

1
Interactions of pig Fab gamma fragments with protein A from Staphylococcus aureus.猪源Fabγ片段与金黄色葡萄球菌蛋白A的相互作用。
Folia Microbiol (Praha). 1980;25(3):246-53. doi: 10.1007/BF02877346.
2
Immunoglobulin M antibodies with ten combining sites.具有十个结合位点的免疫球蛋白M抗体。
Science. 1968 Nov 1;162(3853):574-6. doi: 10.1126/science.162.3853.574.
3
Oxidative sulfitolysis of human IgM.人IgM的氧化亚硫酸解作用
与葡萄球菌蛋白A反应的人多克隆IgE的异质性
Folia Microbiol (Praha). 1984;29(3):264-8. doi: 10.1007/BF02877318.
4
Influence of proteolysis inhibitors and immunoadsorption technique on the composition of rabbit antigen-specific receptor preparations. Presence of Fc receptors.蛋白水解抑制剂和免疫吸附技术对兔抗原特异性受体制剂组成的影响。Fc受体的存在。
Folia Microbiol (Praha). 1983;28(3):205-15. doi: 10.1007/BF02884084.
J Immunol. 1971 Apr;106(4):1136-8.
4
Recognition of two distinct groups of human IgM and IgA based on different binding to staphylococci.基于与葡萄球菌的不同结合识别出两类不同的人IgM和IgA。
Scand J Immunol. 1974;3(4):471-82. doi: 10.1111/j.1365-3083.1974.tb01280.x.
5
Peptic digestion of pig IgM.猪免疫球蛋白M的胃蛋白酶消化作用。
Immunochemistry. 1974 Mar;11(3):115-8. doi: 10.1016/0019-2791(74)90208-0.
6
J chain in pig immunoglobulins.猪免疫球蛋白中的J链。
Immunochemistry. 1973 May;10(5):351-4. doi: 10.1016/0019-2791(73)90083-9.
7
Studies on the structural variation of the H chain of immunoglobulin M(IgM).免疫球蛋白M（IgM）重链结构变异的研究。
Arch Biochem Biophys. 1969 May;131(2):551-60. doi: 10.1016/0003-9861(69)90429-9.
8
The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.蛋白质与戊二醛的交联及其在免疫吸附剂制备中的应用。
Immunochemistry. 1969 Jan;6(1):53-66. doi: 10.1016/0019-2791(69)90178-5.
9
Protein A reactivity of two distinct groups of human monoclonal IgM.两组不同的人源单克隆IgM的蛋白A反应性
Scand J Immunol. 1975;4(8):843-8. doi: 10.1111/j.1365-3083.1975.tb03726.x.
10
Binding of 2,4-dinitrophenyl derivatives by the light chain dimer obtained from immunoglobulin A produced by MOPC-315 mouse myeloma.由MOPC - 315小鼠骨髓瘤产生的免疫球蛋白A获得的轻链二聚体对2,4 - 二硝基苯基衍生物的结合作用
Biochemistry. 1976 Jun 29;15(13):2785-90. doi: 10.1021/bi00658a013.