利用琼脂糖连接的免疫吸附剂分离猪初乳免疫球蛋白并制备抗γ、抗α和抗μ链单特异性抗体。

Isolation of porcine colostral immunoglobulins and preparation of monospecific anti-gamma, anti-alpha, and anti-mu chain antibodies using agarose-linked immunosorbents.

作者信息

Reyero C, Stockl W

出版信息

J Immunol Methods. 1977;15(3):211-21. doi: 10.1016/0022-1759(77)90058-8.

Abstract

A simple method for the simultaneous isolation of IgG2, IgG1, IgA and IgM from porcine colostral whey is described. It makes use of DEAE-cellulose chromatography followed by repeated Sepharose 6B exclusion chromatography after the removal of fat, casein and lipoproteins, Fused rocket immunoelectrophoresis is used to show the elution patterns of each immunoglobulin class. In addition, the prepation of monospecific anti-gamma, anti-alpha and anti-mu sera by means of agarose-linked immunoadsorbents is also described.

摘要

本文描述了一种从猪初乳乳清中同时分离IgG2、IgG1、IgA和IgM的简单方法。该方法利用DEAE-纤维素色谱，在去除脂肪、酪蛋白和脂蛋白后，再进行重复的琼脂糖凝胶6B排阻色谱。采用融合火箭免疫电泳来显示每种免疫球蛋白类别的洗脱模式。此外，还描述了通过琼脂糖连接的免疫吸附剂制备单特异性抗γ、抗α和抗μ血清的方法。