Gokhale V, Mulherkar L
Exp Cell Biol. 1980;48(6):467-72. doi: 10.1159/000163013.
In earlier studies it was found that the effects of urethane could be reversed by folic acid and thymidine but not by uridine. It was further shown that urethane-treated chick embryos were unable to convert serine to glycine, homocysteine to methionine and uridine to thymidine, all of which are folate-dependent reactions. Due to the sensitivity of folic acid auxotrophs towards small amounts of folates present in the biological material, the folic acid contents of urethane-trewated and untreated chick embryos are studied, using microbial assay. It is found that there is a statistically significant reduction in the total folate content of chick embryos treated with urethane as compared to those from the untreated controls. It is conluded that urethane has an antifolate action.
在早期研究中发现，氨基甲酸乙酯的作用可被叶酸和胸苷逆转，但不能被尿苷逆转。进一步研究表明，经氨基甲酸乙酯处理的鸡胚无法将丝氨酸转化为甘氨酸、将同型半胱氨酸转化为蛋氨酸以及将尿苷转化为胸苷，所有这些都是依赖叶酸的反应。由于叶酸营养缺陷型对生物材料中存在的少量叶酸敏感，因此使用微生物测定法研究了经氨基甲酸乙酯处理和未处理的鸡胚中的叶酸含量。结果发现，与未处理的对照组相比，经氨基甲酸乙酯处理的鸡胚总叶酸含量有统计学上的显著降低。得出的结论是，氨基甲酸乙酯具有抗叶酸作用。
