O2 consumption and CO2 influence on the pH and electroretinogram of isolated retina of goldfish.

From micropolarographic O2 measurements, it can be concluded that the isolated goldfish retina is sufficiently supplied with oxygen when 100% O2 is blown over the retina. The retinal O2 consumption amounts to about 60 microliters of O2 per milligram dry weight per hour. Small amounts (0.25% to 2.0%) of CO2 added to the O2 gas diminish the retinal pH, which is 7.0 to 7.1 in situ and after isolation. Moreover, CO2 strongly reduces the ERG amplitude.