利用气相色谱-质谱联用技术对铜绿假单胞菌及相关菌种的挥发性代谢产物进行顶空分析。
Headspace analysis of volatile metabolites of Pseudomonas aeruginosa and related species by gas chromatography-mass spectrometry.
作者信息
Labows J N, McGinley K J, Webster G F, Leyden J J
出版信息
J Clin Microbiol. 1980 Oct;12(4):521-6. doi: 10.1128/jcm.12.4.521-526.1980.
Abstract
Gas chromatographic-mass spectrometric analysis of headspace volatiles was performed on cultures of 11 strains of Pseudomonas aeruginosa and 1 strain each of Pseudomonas cepacia, Pseudomonas putida, Pseudomonas putrefaciens, Pseudomonas fluorescens, and Pseudomonas maltophilia. All strains of Pseudomonas aeruginosa produced a distinctive series of odd-carbon methyl ketones, particularly 2-nonanone and 2-undecanone, and 2-aminoacetophenone. The other strains failed to produce 2-aminoacetophenone. Two sulfur compounds, dimethyldisulfide and dimethyltrisulfide, were present in strains of P. aeruginosa and in variable amounts in other species. Butanol, 2-butanone, 1-undecene, and isopentanol were also detected in P. aeruginosa cultures.
摘要
对11株铜绿假单胞菌以及洋葱伯克霍尔德菌、恶臭假单胞菌、腐败假单胞菌、荧光假单胞菌和嗜麦芽窄食单胞菌各1株的培养物进行了顶空挥发物的气相色谱 - 质谱分析。所有铜绿假单胞菌菌株都产生了一系列独特的奇数碳甲基酮，特别是2 - 壬酮和2 - 十一酮，以及2 - 氨基苯乙酮。其他菌株未能产生2 - 氨基苯乙酮。两种硫化合物，二甲基二硫和二甲基三硫，存在于铜绿假单胞菌菌株中，在其他物种中含量不等。在铜绿假单胞菌培养物中还检测到了丁醇、2 - 丁酮、1 - 十一碳烯和异戊醇。
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