Wade T J, Mandle R J
Appl Microbiol. 1974 Feb;27(2):303-11. doi: 10.1128/am.27.2.303-311.1974.
Strains of saccharolytic and nonsaccharolytic Pseudomonas species were examined by a new single-step gas chromatographic characterization procedure. Cells were digested in a methanolic solution of tetramethylammonium hydroxide pentahydrate, and the digestates were subjected to gas-liquid chromatographic analysis. The chromatograms were examined for similarities and differences in their overall patterns. A single component was defined for use as an internal qualitative and quantitative standardizing component in order to develop relative retention time-relative peak height profiles of each organism. Comparison of these profiles enabled the characterization of strains of Pseudomonas aeruginosa, P. putida, P. cepacia, P. pseudomallei, P. stutzeri, P. pseudoalcaligenes, P. alcaligenes, P. diminuta, P. denitrificans, and P. acidovorans. The P. maltophilia and P. putrefaciens digestates showed chromatograms which were superficially similar yet easily distinguished as belonging to different species. The chromatograms of these two organisms were very different from those of other pseudomonads.
采用一种新的单步气相色谱表征方法对解糖型和非解糖型假单胞菌菌株进行了检测。将细胞在含有五水氢氧化四甲铵的甲醇溶液中消化，然后对消化产物进行气液色谱分析。检查色谱图的整体模式中的异同。定义了一种单一成分用作内部定性和定量标准化成分，以便建立每种微生物的相对保留时间-相对峰高图谱。这些图谱的比较能够对铜绿假单胞菌、恶臭假单胞菌、洋葱伯克霍尔德菌、类鼻疽假单胞菌、施氏假单胞菌、类产碱假单胞菌、产碱假单胞菌、缺陷假单胞菌、反硝化假单胞菌和食酸假单胞菌的菌株进行表征。嗜麦芽窄食单胞菌和腐败假单胞菌的消化产物显示出表面上相似但很容易区分属于不同物种的色谱图。这两种微生物的色谱图与其他假单胞菌的色谱图非常不同。
