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铜绿假单胞菌O抗原的测定与鉴定(作者译)

[O-antigen determination and identification of Pseudomonas aeruginosa (author's transl)].

作者信息

Ansorg R

出版信息

Zentralbl Bakteriol A. 1980 Mar;246(2):197-203.

PMID:6775439
Abstract

The quality of the morphological-biochemical identification of P. aeruginosa affects the results of group determination with anti-O-sera (Institute Pasteur Production) to a great extent. Out of 563 isolates classified as P. aeruginosa on the basis of colony morphology, haemolysis, pigment formation, odor, gramstaining and oxidase test, 84,2% react with one monovalent antiserum. Out of 498 strains which are more exactly identified by additional use of the Oxi/Ferm tube-system (Hoffmann-La Roche), 94,0% are clearly to be grouped. The O-antigen determination is not appropriate for inter-species differentiation of P. aeruginosa, due to common antigens with P. aeruginosa related species.

摘要

铜绿假单胞菌形态生化鉴定的质量在很大程度上影响使用抗O血清(巴斯德研究所生产)进行分组测定的结果。在563株根据菌落形态、溶血、色素形成、气味、革兰氏染色和氧化酶试验归类为铜绿假单胞菌的分离株中,84.2%与一种单价抗血清发生反应。在通过额外使用Oxi/Ferm管系统(霍夫曼-罗氏公司)更准确鉴定的498株菌株中,94.0%能够明确分组。由于与铜绿假单胞菌相关菌种存在共同抗原,O抗原测定不适用于铜绿假单胞菌的种间鉴别。

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