Gel electrophoresis and glass permeation chromatography of the hamster scrapie agent after enzymatic digestion and detergent extraction.

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作者信息

Prusiner S B, Groth D F, Cochran S P, McKinley M P, Masiarz F R

出版信息

Biochemistry. 1980 Oct 14;19(21):4892-8. doi: 10.1021/bi00562a029.

DOI:10.1021/bi00562a029

PMID:6775698

Abstract

摘要

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Gel electrophoresis and glass permeation chromatography of the hamster scrapie agent after enzymatic digestion and detergent extraction.

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Investigating the conformational stability of prion strains through a kinetic replication model.通过动力学复制模型研究朊病毒株的构象稳定性。

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Etiology and pathogenesis of prion diseases.

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Thiocyanate and hydroxyl ions inactivate the scrapie agent.硫氰酸盐和氢氧根离子可使瘙痒病病原体失活。

Proc Natl Acad Sci U S A. 1981 Jul;78(7):4606-10. doi: 10.1073/pnas.78.7.4606.

Scrapie agent contains a hydrophobic protein.羊瘙痒病病原体含有一种疏水蛋白。

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On the biology of prions.论朊病毒生物学

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Proc Natl Acad Sci U S A. 1990 Sep;87(18):7240-4. doi: 10.1073/pnas.87.18.7240.

Protease sensitivity and nuclease resistance of the scrapie agent propagated in vitro in neuroblastoma cells.在神经母细胞瘤细胞中体外增殖的瘙痒病病原体的蛋白酶敏感性和核酸酶抗性

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