Stöffler G, Cundliffe E, Stöffler-Meilicke M, Dabbs E R
J Biol Chem. 1980 Nov 10;255(21):10517-22.
Three mutants with ribosomes apparently lacking Protein L11, AM68, AM76, and AM77, were investigated using a variety of immunological techniques to determine whether L11 was indeed lacking. Ouchterlony double diffusion, modified immunoelectrophoresis, and dimer formation on sucrose gradients all gave results indicating Protein L11 was missing from the ribosome in these mutants. Electron micrographs of ribosomes of the mutants were indistinguishable from those of wild type. Ribosomes of AM68, AM76, and AM77, did not bind the antibiotic thiostrepton, but binding was recovered upon reconstitution with wild type Protein L11.
利用多种免疫学技术对三个核糖体明显缺乏L11蛋白的突变体AM68、AM76和AM77进行了研究，以确定L11是否确实缺失。双向琼脂扩散试验、改良免疫电泳以及蔗糖梯度上的二聚体形成实验所得结果均表明，这些突变体的核糖体中缺少L11蛋白。突变体核糖体的电子显微镜照片与野生型核糖体的照片没有区别。AM68、AM76和AM77的核糖体不结合抗生素硫链丝菌素，但用野生型L11蛋白重建后可恢复结合。
