Warn R M, Bullard B, Magrath R
J Embryol Exp Morphol. 1980 Jun;57:167-76.
Changes in the distribution of myosin during the formation of the cellular blastoderm of Drosophila melanogaster were followed by staining sections of embryos with antibodies to myosin. These were visualized with indirect immunofluorescence. Prior to the start of cell membrane extension myosin is distributed between the nuclear caps as a thin sub-plasmalemma layer. There is also myosin present beneath the surface of the caps. When plasmalemma growth occurs, myosin is associated with the furrow canals, the tips of the advancing membranes. The fluorescence is distributed in an approximately hexagonal pattern around the growth points of each cell. The hexagons are joined up forming a network. It is suggested that this myosin is associated with bundles of microfilaments, orientated parallel to the surface, to form many interlocking contractile rings. The simultaneous contraction of these rings causes the cleavage of the blastoderm. During the first phase of membrane growth, myosin is also associated with the apical surfaces of the forming cells. At this stage these surfaces are rich in microvilli. However, by the time the furrow canals have reached the bases of the cells much of this myosin has disappeared. At about this time the apical surface becomes taut with a loss of the microvilli.
通过用抗肌球蛋白抗体对果蝇胚胎切片进行染色，追踪了黑腹果蝇细胞胚盘形成过程中肌球蛋白分布的变化。这些通过间接免疫荧光进行可视化观察。在细胞膜开始延伸之前，肌球蛋白作为一层薄的亚质膜层分布在核帽之间。在核帽表面下方也存在肌球蛋白。当质膜生长时，肌球蛋白与沟道相关联，沟道位于前进膜的尖端。荧光围绕每个细胞的生长点以近似六边形的模式分布。六边形相互连接形成一个网络。有人提出，这种肌球蛋白与平行于表面排列的微丝束相关联，形成许多相互连锁的收缩环。这些环的同时收缩导致胚盘的分裂。在膜生长的第一阶段，肌球蛋白也与正在形成的细胞的顶端表面相关联。在这个阶段，这些表面富含微绒毛。然而，当沟道到达细胞底部时，大部分这种肌球蛋白已经消失。大约在这个时候，顶端表面变得紧绷，微绒毛消失。
