Shklar G, Flynn E, Szabo G, Marefat P
J Natl Cancer Inst. 1980 Dec;65(6):1307-16.
Sixty-four male and female Syrian golden hamsters (Mesocricetus auratus) were divided into 4 equal groups. Group 1 animals had the posterior lateral borders of their tongues painted three times weekly with a 0.5% solution of 7,12-dimethylbenz[a]anthracene (DMBA) in acetone. Group 2 animals were painted with DMBA and also received 10 mg of 13-cis-retinoic acid in peanut oil administered orally by pipette twice weekly. Group 3 animals received only retinoid, and group 4 animals were untreated controls. Four animals in each group were killed at 12, 14, 16, and 18 weeks. In the DMBA-painted animals receiving 13-cis-retinoic acid, the leukoplakia and epidermoid carcinomas developed more slowly and were better differentiated microscopically. The better degree of differentiation was demonstrated by ultrastructural studies. Less cellular separation and fewer microvilli-like structures were evident. No tubular mitochondria were observed. Less dispersion of heterochromatin occurred throughout nuclei, and the basal lamina tended to be regular and continuous.
64只叙利亚金黄地鼠（Mesocricetus auratus），雌雄各半，被分为4组，每组数量相等。第1组动物的舌后外侧边缘每周用含0.5% 7,12-二甲基苯并[a]蒽（DMBA）的丙酮溶液涂抹3次。第2组动物涂抹DMBA，同时每周用移液管经口给予10毫克花生油溶解的13-顺式维甲酸2次。第3组动物仅接受类维生素A，第4组动物为未处理的对照组。每组4只动物在第12、14、16和18周处死。在接受13-顺式维甲酸的DMBA涂抹动物中，白斑和表皮样癌发展较慢，显微镜下分化较好。超微结构研究证实了较好的分化程度。细胞分离较少，微绒毛样结构较少。未观察到管状线粒体。整个细胞核中异染色质的分散较少，基膜趋于规则和连续。
