Shklar G
J Natl Cancer Inst. 1982 May;68(5):791-7.
Eighty young adult male and female Syrian golden hamsters (Mesocricetus auratus) were divided into 4 equal experimental groups. In group I animals the left buccal pouch was painted three times weekly with a 0.5% solution of 7,12-dimethylbenz[a]anthracene (DMBA) in heavy mineral oil. In group 2 animals the left buccal pouch was similarly painted with DMBA, but the animals also received orally 10 mg vitamin E (dl-alpha-tocopherol) in peanut oil twice weekly on alternate days with DMBA painting. Group 1 animals received a similar amount of peanut oil vehicle, group 3 animals received only vitamin E (vitamin E controls) in peanut oil, and group 4 animals served as untreated controls receiving only peanut oil. Four animals in each group (2 males and 2 females) were killed at 8, 10, 12, 14, and 16 weeks. Buccal pouches were photographed and excised, and tumors were noted and measured in the left buccal pouches. In group 2 animals receiving vitamin E, tumor formation was significantly delayed, so that by 14-16 weeks there were fewer tumors and their average size was smaller than that of tumors in group 1 animals that were painted with DMBA but received no vitamin E supplement. In group 2 there was also less invasion of underlying tissues and less surface necrosis.
80只成年叙利亚金黄地鼠（Mesocricetus auratus）被分为4个相等的实验组。在第1组动物中，每周用重质矿物油中的0.5% 7,12-二甲基苯并[a]蒽（DMBA）溶液对左侧颊囊涂抹3次。在第2组动物中，左侧颊囊同样用DMBA涂抹，但动物还每周口服两次10毫克花生油中的维生素E（dl-α生育酚），与DMBA涂抹隔日进行。第1组动物接受等量的花生油载体，第3组动物仅接受花生油中的维生素E（维生素E对照组），第4组动物作为未处理对照组，仅接受花生油。每组4只动物（2只雄性和2只雌性）在8、10、12、14和16周时处死。拍摄颊囊照片并切除，记录并测量左侧颊囊中的肿瘤。在接受维生素E的第2组动物中，肿瘤形成明显延迟，因此到14 - 16周时，肿瘤数量减少，其平均大小小于仅涂抹DMBA但未补充维生素E的第1组动物中的肿瘤。在第2组中，对深层组织的侵犯也较少，表面坏死也较少。
