大肠杆菌苯丙氨酸转运核糖核酸（tRNA Phe）一种新型、具有完全活性的荧光衍生物的表征
Characterisation of a new, fully active fluorescent derivative of E. coli tRNA Phe.
作者信息
Plumbridge J A, Bäumert H G, Ehrenberg M, Rigler R
出版信息
Nucleic Acids Res. 1980 Feb 25;8(4):827-43.
Abstract
E. coli tRNAPhe has been labelled with fluorescein isothiocyanate taking advantage of the reactivity of this compound for primary aliphatic amino groups as exist in this tRNA as the modified base X(3-(3-amino-3-carboxypropyl)uracil). The extent of labelling was calculated as 1.6 nmole/A260 unit suggesting one dye molecule per tRNA. The FITC-tRNA showed full activity in aminoacylation and polypeptide synthesis. The absorption and fluorescence of the label respond markedly on addition of Mg++ to the tRNA. The label appears to be a sensitive probe of tRNAPhe tertiary structure.
摘要
利用异硫氰酸荧光素与一级脂肪族氨基的反应活性，已将其标记到大肠杆菌苯丙氨酸转运核糖核酸（tRNAPhe）上，该一级脂肪族氨基以修饰碱基X（3 -（3 -氨基- 3 -羧丙基）尿嘧啶）的形式存在于这种转运核糖核酸中。标记程度经计算为1.6纳摩尔/A260单位，表明每个转运核糖核酸有一个染料分子。异硫氰酸荧光素标记的转运核糖核酸（FITC - tRNA）在氨酰化和多肽合成中表现出完全活性。向转运核糖核酸中添加镁离子后，标记物的吸收和荧光有明显反应。该标记物似乎是tRNAPhe三级结构的灵敏探针。
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Characterisation of a new, fully active fluorescent derivative of E. coli tRNA Phe.大肠杆菌苯丙氨酸转运核糖核酸（tRNA Phe）一种新型、具有完全活性的荧光衍生物的表征
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