Synthesis of newer N-furylidene and N-acetophenonylidene-5-chlorodiphenylamine-2-carboxylic acid hydrazides as antiviral and antibacterial agents.

Five N-furylidene and fifteen N-acetophenonylidene-5-chlorodiphenylamine-2-carboxylic acid hydrazides were synthesized by the condensation of 5-chlorodiphenylamine-2-carboxylic acid hydrazides with furfuraldehyde and appropriate acetophenones. All the N-acetophenonylidenes thus synthesized were screened for their antiviral activity against animal viruses (Ranikhet disease virus and vaccinia virus in a stationary culture of chorioallantoic membrane of chick embryo) and against plant virus, namely gomphrena mosaic virus (GMV) in vitro as well as in vivo. Whereas five N-furylidenes were tested against gomphrena mosaic virus only in vitro and in vivo for their antiviral activity. Maximum significant protection was observed to the extent of 20% against Ranikhet disease virus. The majority of these compounds showed highly significant antiviral activity up to the extent of 96% in vitro and 89% in vivo against gomphrena mosaic virus. These compounds were also screened for their antibacterial activity against two micro-organisms; Bacillus subtilis and Staphylococcus aureus. Almost all of them were found to exhibit significant inhibition against B. subtilis while only a fewer showed significant inhibition against S. aureus.